Biomechanical, biochemical and structural correlations in immature and mature rabbit articular cartilage
Biomechanical, biochemical and structural correlations in immature and mature rabbit articular cartilage
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DOI：
10.1016/j.joca.2009.07.002
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发表时间：
2009-12-01
影响因子：
7
通讯作者：
Helminen, H. J.
中科院分区：
文献类型：
--
作者：
Julkunen, P.;Harjula, T.;Helminen, H. J.
目的：关节软骨在发育和生长过程中结构和成分发生变化。这些变化导致软骨机械性能的改变。本研究旨在探讨兔关节软骨在生长和成熟过程中的生物力学、生物化学和结构的关系，实验设计：取雌性新西兰白色兔胫骨内侧平台和股骨内侧髁的关节软骨标本，分7个年龄组进行; 0天（n=29）、11天（n= 0）、4周（n=30）、6周（n= 30）、3个月（n = 24）、6个月（n=24）和18个月（n=19）。在蠕变压痕下对样品进行机械测试。从机械响应，瞬时和平衡模量被确定。对全层软骨样品中的组织胶原、羟赖氨酰吡啶啉（HP）和戊糖苷（PEN）交联进行生化分析。通过测量番红O染色样品的光密度，从组织切片的深度方向研究蛋白聚糖。此外，关节软骨的胶原结构的深度方向进行了分析与偏振光显微镜。有限元分析的样品从不同的年龄组进行揭示的纤维网络和关节软骨基质的拉伸和压缩性能，respectively，Results：组织厚度从类似3到类似0.5毫米，直到3个月的年龄下降，而瞬时模量随着年龄的增加之前的峰值在4-6周。3月龄前平衡模量较低，3月龄后平衡模量继续增加。胶原原纤维取向角和平行度指数与瞬时模量、拉伸原纤维模量和组织厚度呈负相关。胶原含量和交联呈正相关的平衡压缩properties of the tissue.Conclusions：在成熟过程中，组织结构，组成和机械性能的显着调制发生。重要的是，本研究提供了深入了解机械，化学和结构的相互作用，导致成熟的关节软骨的功能特性。(C)2009年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: The structure and composition of articular cartilage change during development and growth. These changes lead to alterations in the mechanical properties of cartilage. In the present study, biomechanical, biochemical and structural relationships of articular cartilage during growth and maturation of rabbits are investigated.Design: Articular cartilage specimens from the tibial medial plateaus and femoral medial condyles of female New Zealand white rabbits were collected from seven age-groups; 0 days (n=29), 11 days (n= 0), 4 weeks (n=30), 6 weeks (n=30), 3 months (n = 24), 6 months (n=24) and 18 months (n=19). The samples underwent mechanical testing under creep indentation. From the mechanical response, instantaneous and equilibrium moduli were determined. Biochemical analyses of tissue collagen, hydroxylysylpyridinoline (HP) and pentosidine (PEN) cross-links in full thickness cartilage samples were conducted. Proteoglycans were investigated depth-wise from the tissue sections by measuring the optical density of Safranin-O-stained samples. Furthermore, depth-wise collagen architecture of articular cartilage was analyzed with polarized light microscopy. Finite element analyses of the samples from different age-groups were conducted to reveal tensile and compressive properties of the fibril network and the matrix of articular cartilage, respectively.Results: Tissue thickness decreased from similar to 3 to similar to 0.5 mm until the age of 3 months, while the instantaneous modulus increased with age prior to peak at 4-6 weeks. A lower equilibrium modulus was observed before 3-month-age, after which the equilibrium modulus continued to increase. Collagen fibril orientation angle and parallelism index were inversely related to the instantaneous modulus, tensile fibril modulus and tissue thickness. Collagen content and cross-linking were positively related to the equilibrium compressive properties of the tissue.Conclusions: During maturation, significant modulation of tissue structure, composition and mechanical properties takes place. Importantly, the present study provides insight into the mechanical, chemical and structural interactions that lead to functional properties of mature articular cartilage. (C) 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.