Lanthanide-Labeled Immunochromatographic Strip Assay for the On-Site Identification of Ancient Silk
Lanthanide-Labeled Immunochromatographic Strip Assay for the On-Site Identification of Ancient Silk
复制标题
稀土标记免疫层析试纸条检测用于古代丝绸的现场鉴定
DOI：
10.1021/acssensors.7b00086
复制
发表时间：
2017-04-01
期刊：
影响因子：
8.9
通讯作者：
Wang, Bing
中科院分区：
文献类型：
--
作者：
You, Qiushi;Liu, Miaomiao;Wang, Bing
古代丝绸的现场鉴定长期以来一直是考古学的一个关键挑战。因此，一种快速、经济、灵敏的分析方法是非常必要的。本文介绍了一种适合于古丝绸现场鉴定的稀土标记免疫层析纸条。与传统的胶体金免疫层析纸条相比，该纸条具有更高的分析灵敏度和更好的定量鉴别能力。该试条对丝素蛋白(SF)的检出限为8.09 ng/mL，约为胶体金免疫层析试纸条的185倍。未观察到与其他可能的干扰抗原的交叉反应。重现性好，重复性好，平均回收率为94.15-102.55%，变异系数为5.22-17.57%。基于可携带性和成本效益的优势，以及灵敏度、特异性和重复性，稀土标记的免疫层析纸条是一种在考古野外工作中现场检测古代文物的有前途的工具。
The on-site identification of ancient silks has long been a key challenge in archeology. Therefore, a rapid, cost-effective, sensitive analytical approach is highly desirable. In this paper, a lanthanide-labeled immunochromatographic strip which is suitable for the on-site identification of ancient silks is described. Compared with the conventional colloidal gold-based immunochromatographic strip, this strip shows much higher analytical sensitivity and better quantitative discrimination. The limit of detection (LOD) of the strip for silk fibroin (SF) was calculated as 8.09 ng/mL, approximately 185 times lower than that of the colloidal gold-based immunochromatographic strip. No cross-reactions with other possible interfering antigens were observed. Moreover, the strip also shows good reproducibility, with a mean recovery of 94.15-102.55% and coefficient of variation of 5.22-17.57% in the repeated tests. Based on the advantages of portability and cost-effectiveness, as well as sensitivity, specificity, and reproducibility, the lanthanide-labeled immunochromatographic strip is a promising tool for on-site detection of ancient relics in archeological fieldwork.