Oral-bacterial-induced arterial and venous thrombus in rats: Pathological and immunological studies.
Oral-bacterial-induced arterial and venous thrombus in rats: Pathological and immunological studies.
复制标题
口腔细菌诱导的大鼠动脉和静脉血栓：病理学和免疫学研究。
DOI：
10.1002/cre2.215
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Izumi Y.
中科院分区：
文献类型：
--
作者：
Iwai T;Matsui Y;Homma K;Takemura T;Fujiwara M;Aoyama N;Sato H;Izumi Y.
ObjectivesOur study investigated the pathological outcome of experimental thrombi that incorporate oral bacteria.Material and methodsA small artery and vein in the rats' groins was injected with a solution containing牙周细菌牙龈卟啉单胞菌and followed up for 28 d.总共将9只雄性大鼠（500-650 g）的18条肢体用于动脉研究，将4只大鼠的8条肢体用于静脉研究。在动脉研究中使用了两种密度的细菌溶液和两种动脉厚度尺寸。近端和远端的动脉和静脉结扎松散使用单丝尼龙缝线前细菌悬浮液或对照溶液注入结扎vessels.ResultsAfter 7，14-18，和28天，大鼠被处死。进行病理学和免疫组织化学检查。所有样本在第7天均显示血栓形成和粒细胞急性炎症反应，然后在第28天在动脉血栓中稳定为浆细胞或巨噬细胞慢性纤维性变化。在动脉血栓愈合过程中观察到CD 3（Pan T细胞）、CD 79 a（大鼠中的Pan B细胞）和IgG。静脉变化显示在第7天出现相对明显的再通，这与动脉略有不同。粒细胞存在7至28 day. ConclusionsPeriodontitis细菌作为一个炎症核心的血管，但不是作为一个感染剂，在我们的实验中，因为他们的能力低，侵入组织。
ObjectivesOur study investigated the pathological outcome of experimental thrombi that incorporate oral bacteria.Material and methodsA small artery and vein in the rats' groins were injected with a solution containing periodontal bacteriaPorphyromonas gingivalisand followed up for 28 days. In all, 18 limbs of nine male rats (500–650 g) were used for the arterial study, and eight limbs of four rats were used for the veins. Two densities of the bacterial solution and two arterial thicknesses sizes were used in the arterial study. Both proximal and distal arteries and veins were ligated loosely using a monofilament nylon suture before bacterial suspensions or control solutions were injected into the ligated vessels.ResultsAfter 7, 14–18, and 28 days, the rats were sacrificed. Pathology and immunohistochemistry were performed. All specimens exhibited thrombus formation and an acute inflammation reaction with granulocytes at 7 days and then settled down to chronic fibrous change with plasma cells or macrophages at 28 days in the arterial thrombus. CD3 (Pan T‐cells), CD79a (Pan B cells in the rats), and IgG were observed in the process of the healing of the arterial thrombus. Venous changes showed relatively clear recanalization that appeared at 7 days, which is slightly different from the artery. Granulocytes were present from 7 to 28 days.ConclusionsPeriodontal bacteria act as an inflammatory core in the vessels, but not as an infectious agent, in our experiments, because of their low ability to invade tissues.