Restriction of transient receptor potential vanilloid-1 to the peptidergic subset of primary afferent neurons follows its developmental downregulation in nonpeptidergic neurons.
Restriction of transient receptor potential vanilloid-1 to the peptidergic subset of primary afferent neurons follows its developmental downregulation in nonpeptidergic neurons.
复制标题
DOI：
10.1523/jneurosci.1299-11.2011
复制
发表时间：
2011-07-13
期刊：
影响因子：
--
通讯作者：
Basbaum AI
中科院分区：
文献类型：
--
作者：
Cavanaugh DJ;Chesler AT;Bráz JM;Shah NM;Julius D;Basbaum AI
初级传入“疼痛”纤维（伤害感受器）被划分为不同的分子和解剖特征的基础上的亚类，这些类介导的伤害性模式特异性的贡献引起的疼痛刺激的行为。然而，热和辣椒素受体TRPV1是否在几个感觉群体中不均匀地表达，或者由独特的伤害感受器亚类选择性地表达，尚不清楚。在这里，我们使用了两个线的Trpv1报告小鼠研究TRPV1的初级传入表达，无论是在发展过程中，并在成人。我们使用Cre诱导的谱系追踪证明，在发育过程中，TRPV 1在广泛的背根神经节神经元中短暂表达，并且其表达逐渐细化，使得TRPV 1转录本仅限于肽能感觉神经元的特定子集。最后，这些报告小鼠所特有的显着敏感性揭示了成年TRPV 1+初级传入对中枢和外周靶点的神经支配，其范围比之前认识到的要广泛得多。
Primary afferent “pain” fibers (nociceptors) are divided into subclasses based on distinct molecular and anatomical features, and these classes mediate noxious modality-specific contributions to behaviors evoked by painful stimuli. Whether the heat and capsaicin receptor, TRPV1, is expressed heterogeneously across several sensory populations, or is selectively expressed by a unique nociceptor subclass, however, is unclear. Here we used two lines of Trpv1 reporter mice to investigate the primary afferent expression of TRPV1, both during development and in the adult. We demonstrate, using Cre-induced lineage tracing, that during development, TRPV1 is transiently expressed in a wide range of DRG neurons, and that its expression is gradually refined, such that TRPV1 transcripts become restricted to a specific subset of peptidergic sensory neurons. Finally, the remarkable sensitivity that is characteristic of these reporter mice revealed an innervation of central and peripheral targets by TRPV1+ primary afferents in the adult that is considerably more extensive than has previously been appreciated.