Study of KIR gene expression at the mRNA level in specific donor-derived NK cells after allogeneic HSCT
Study of KIR gene expression at the mRNA level in specific donor-derived NK cells after allogeneic HSCT
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同种异体 HSCT 后特定供体来源的 NK 细胞中 KIR 基因在 mRNA 水平表达的研究
DOI：
10.1007/s00251-019-01153-6
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发表时间：
2020-04-01
期刊：
影响因子：
3.2
通讯作者：
He, Jun
中科院分区：
文献类型：
--
作者：
Li, Ying;Wang, Tian;He, Jun
同种异体造血干细胞移植（alloo - hsct）后自然杀伤细胞（NK）的功能受抑制型KIRs （iKIRs）和激活型KIRs （aKIRs）之间的平衡调节。然而，很少有研究检查了供体特有的KIR基因的后续表达。我们定义了仅在供体中表达的KIR基因集，并设计了一种基于基因克隆技术的实时定量聚合酶链反应（RT-qPCR）检测这些KIR基因表达的方法。在这项研究中，我们评估了252对供体-受体中KIR基因的恢复模式。每个供体独有的KIR表达与供体和受体共有的KIR基因表达一致，如KIR2DS1、KIR3DS1、KIR2DS4或KIR2DS3。供体独有的aKIRs mRNA表达高峰的时间不一致，但发生在移植后的前3个月内，而在移植后的第3个月一致观察到kirs mRNA表达高峰。KIR2DL2在移植后第3个月的表达在受体中明显高于供体（p= 0.01）。移植后2、3个月受者KIR2DL1、KIR3DL1水平明显高于供者（p< 0.0001）。因此，在试图预测同种异体造血干细胞移植后mRNA表达与预后之间的相关性时，应考虑这些观察结果。
The function of natural killer (NK) cells after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is regulated by the balance between inhibitory KIRs (iKIRs) and activating KIRs (aKIRs). However, few studies have examined the subsequent expression of KIR genes unique to the donor. We defined the set of KIR genes expressed only in the donor and designed a method for measuring the expression of these KIR genes by quantitative real-time polymerase chain reaction (RT-qPCR) based on genetic cloning techniques. In this study, we evaluated the recovery pattern of KIR genes in 252 donor-recipient pairs. The expression of each KIR unique to the donor was in line with that of KIR genes shared by the donor and recipient, such as KIR2DS1, KIR3DS1, KIR2DS4, or KIR2DS3. The timing of the peak mRNA expression of aKIRs unique to the donor was inconsistent but occurred within the first 3 months posttransplantation, whereas the peak mRNA expression of iKIRs was consistently observed in the third month after transplantation. The expression of KIR2DL2 in the third month posttransplantation was significantly higher in the transplant recipients than in the donors (p= 0.01). The KIR2DL1 and KIR3DL1 levels in the transplant recipients in the second and third months posttransplantation were also obviously higher than the donor levels (p< 0.0001). Thus, these observations should be considered when attempting to predict the correlation between mRNA expression and prognosis after allo-HSCT.