A SUMO ligase AtMMS21regulates the stability of the chromatin remodeler BRAHMA in root development
A SUMO ligase AtMMS21regulates the stability of the chromatin remodeler BRAHMA in root development
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SUMO 连接酶 AtMMS21 调节染色质重塑剂 BRAHMA 在根发育中的稳定性。
DOI：
10.1104/pp.17.00014
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发表时间：
2017
期刊：
影响因子：
7.4
通讯作者：
Chengwei Yang
中科院分区：
文献类型：
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作者：
Juanjuan Zhang;Jianbin Lai;Feige Wang;Songguang Yang;Zhipeng He;Jieming Jiang;Qingliang Li;Qian Wu;Yiyang Liu;Mengyuan Yu;Jinju Du;Qi Xie;Keqiang Wu;Chengwei Yang
染色质重塑是植物发育和逆境应答过程中基因表达调控的关键。Brahma （BRM）是SWI/SNF染色质重塑复合体中的一种保守的atp酶，参与植物细胞的多种生物过程，但BRM蛋白的调控机制尚不清楚。在这里，我们报道了BRM与拟南芥（拟南芥）中的SUMO连接酶AtMMS21相互作用。这种相互作用在体内和体外的不同方法中得到证实。突变体brmandatmms21在根发育中也表现出类似的缺陷。在突变体中，BRM-GFP蛋白水平显著低于野生型，但brm的RNA水平没有变化。生化证据表明，BRM被SUMO3修饰，AtMMS21增强了反应。此外，过表达野生型AtMMS21而不表达没有SUMO连接酶活性的突变AtMMS21能够恢复BRM inmms21-1的稳定性。brminmms21 - 11的过表达部分挽救了根的发育缺陷。综上所述，这些结果支持AtMMS21调控BRM在根系发育过程中的蛋白稳定性。
Chromatin remodeling is essential for gene expression regulation in plant development and response to stresses. Brahma (BRM) is a conserved ATPase in the SWI/SNF chromatin remodeling complex and is involved in various biological processes in plant cells, but the regulation mechanism on BRM protein remains unclear. Here, we report that BRM interacts with AtMMS21, a SUMO ligase in Arabidopsis (Arabidopsis thaliana). The interaction was confirmed in different approaches in vivo and in vitro. The mutants ofBRMandAtMMS21displayed a similar defect in root development. In themms21-1mutant, the protein level of BRM-GFP was significantly lower than that in wild type, but the RNA level ofBRMdid not change. Biochemical evidence indicated that BRM was modified by SUMO3, and the reaction was enhanced by AtMMS21. Furthermore, overexpression of wild-type AtMMS21 but not the mutated AtMMS21 without SUMO ligase activity was able to recover the stability of BRM inmms21-1. Overexpression ofBRMinmms21-1partially rescued the developmental defect of roots. Taken together, these results supported that AtMMS21 regulates the protein stability of BRM in root development.