p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers
p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers
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DOI：
10.1016/s0964-1955(97)00027-4
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发表时间：
1997-07-01
期刊：
影响因子：
4.8
通讯作者：
Mothersill, C
中科院分区：
文献类型：
--
作者：
Colucci, S;ElGehani, R;Mothersill, C
对77例已知吸烟或饮酒史的正常口腔黏膜进行培养。培养14-21天后，用免疫组织化学染色上皮细胞检测p53表达。对这些样本的随机子集（20名不吸烟者和21名吸烟者）进行了p53基因的PCR-SSCP分析。经p53-240抗体检测，g53蛋白稳定、无功能形式的表达在吸烟者培养的口腔黏膜中显著升高(P
The normal oral mucosa from 77 individuals of known smoking sand alcophol-intake history was cultured. After 14-21 days in culture, epithelial cells were stained for p53 expression using immunohistochemistry. PCR-SSCP analysis of the p53 gene was also performed on a random sub-set of these samples (20 non-smokers and 21 smokers). Expression of the stable, non-functional form of g53 protein as detected by the p53-240 antibody was found to be significantly elevated in the cultured oral mucosa of smokers (P