CORPUS-LUTEUM ANGIOGENIC FACTOR IS RELATED TO FIBROBLAST GROWTH-FACTOR
CORPUS-LUTEUM ANGIOGENIC FACTOR IS RELATED TO FIBROBLAST GROWTH-FACTOR
复制标题
DOI：
10.1210/endo-117-6-2383
复制
发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
BOHLEN, P
中科院分区：
文献类型：
--
作者：
GOSPODAROWICZ, D;CHENG, J;BOHLEN, P
通过差示盐沉淀、离子交换层析和肝素-琼脂糖层析的组合，牛黄体 (CL) 中存在的血管生成生长因子已被纯化至明显均质。它是一种单链多肽，表观分子量为 15,000，氨基酸组成与之前报道的垂体和脑成纤维细胞生长因子 (FGF) 相似。 CL衍生生长因子的前17个残基的序列分析鉴定出序列：His-Phe-Lys-Asp-Pro-Lys-Arg-Leu-Tyr-×。 -Lys-Asn-Gly-Gly- .次。 -苯丙氨酸-亮氨酸。该序列与牛垂体和脑 FGF 的残基 16-33 相同，表明 Cl 衍生的生长因子是 FGF 的氨基末端截短形式，并且在其他方面与 FGF 相似（即使不相同）。 CL FGF 的生物活性与垂体或脑 FGF 的生物活性没有区别。它在触发源自大血管（主动脉弓）或黄体和肾上腺皮质毛细血管的培养牛血管内皮细胞的增殖方面具有高度活性（20-40 pg/ml 的半最大刺激和 400-600 pg/ml 的饱和度）。含有 50 ng 至 1 μg CL 衍生生长因子的体内植入物刺激鸡胚绒毛尿囊膜中的新血管形成。除了促进血管内皮细胞有丝分裂外，CL FGF 还刺激多种中胚层和神经外胚层衍生细胞的增殖，包括血管平滑肌细胞、颗粒细胞和肾上腺皮质细胞、兔肋软骨细胞和角膜内皮细胞。
An angiogenic growth factor present in bovine corpus luteum (CL) has been purified to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, and heparin-Sepharose chromatography. It is a single chain polypeptide with an apparent mol wt of 15,000 and an amino acid composition similar to that previously reported for pituitary and brain fibroblast growth factor (FGF). Sequence analysis of the first 17 residues of the CL-derived growth factor identified the sequence: His-Phe-Lys-Asp-Pro-Lys-Arg-Leu-Tyr- .times. -Lys-Asn-Gly-Gly- .times. -Phe-Leu. This sequence is identical to residues 16-33 of bovine pituitary and brain FGF, indicating that the Cl-derived growth factor is an amino-terminally truncated form of FGF and is otherwise similar, if not identical, to FGF. The biological activity of CL FGF is indistinguishable from that of pituitary or brain FGF. It is highly active in triggering the proliferation of cultured bovine vascular endothelial cells derived either from large vessels (aortic arch) or from corpus luteum and adrenal cortex capillaries (half-maximal stimulation at 20-40 pg/ml and saturation at 400-600 pg/ml). In vivo implants containing 50 ng to 1 .mu.g CL-derived growth factor stimulate neovascularization in the chorioallantoic membrane of the chick embryo. In addition to being mitogenic for vascular endothelial cells, CL FGF also stimulates the proliferation of a wide variety of mesoderm- and neuroectoderm-derived cells, including vascular smooth muscle cells, granulosa and adrenal cortex cells, rabbit costal chondrocytes, and corneal endothelial cells.