Expression and cell type--specific processing of human preproenkephalin with a vaccinia recombinant.
Expression and cell type--specific processing of human preproenkephalin with a vaccinia recombinant.
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表达和细胞类型——用痘苗重组体对人前脑啡肽原进行特异性加工。
DOI：
10.1126/science.3754979
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hruby,DE
中科院分区：
文献类型：
--
作者：
Thomas,G;Herbert,E;Hruby,DE
通过用表达人脑啡肽原的重组痘苗病毒感染多种细胞类型来评估复杂前体多蛋白（人脑啡肽原）的翻译后成熟。受感染的细胞迅速产生细胞和分泌的甲硫氨酸脑啡肽免疫反应性。尽管每种细胞系都可以分泌完整的脑啡肽原，但只有小鼠垂体细胞系能够将脑啡肽原加工成成熟的脑啡肽。 BSC-40 细胞（源自非洲绿猴肾）分泌的完整脑啡肽原的数量足以确立痘苗病毒作为哺乳动物细胞表达载体的价值。
The posttranslational maturation of a complex precursor polyprotein, human proenkephalin, was assessed by infection of a wide spectrum of cell types with a recombinant vaccinia virus that expressed human proenkephalin. The infected cells rapidly produced both cellular and secreted Met-enkephalin immunoreactivity. Although each cell line could secrete intact proenkephalin, only a mouse pituitary line was capable of processing proenkephalin to mature enkephalin peptides. The quantity of intact proenkephalin secreted from BSC-40 cells (derived from African Green monkey kidney) was sufficient to establish the value of vaccinia virus as a mammalian cell expression vector.