p53 Mutations in human hepatocellular carcinomas from Germany.
p53 Mutations in human hepatocellular carcinomas from Germany.
复制标题
德国人肝细胞癌中的 p53 突变。
作者：
S. Kress;U. Jahn;A. Buchmann;P. Bannasch;M. Schwarz
p53基因突变是人类肝细胞癌中常见的遗传学改变。我们研究了13例人肝细胞癌从德国的p53基因外显子4至8的存在畸变的单链构象多态性和限制性片段长度多态性分析和聚合酶链反应产物测序。在两个人肝细胞癌中发生了单碱基替换：密码子257的CpG位点发生C：G-T：A转换，密码子273发生T：A-A：T颠换。这些点突变的肿瘤和两个额外的肿瘤没有点突变表现出一个p53等位基因的损失。所有肿瘤均未发生c-Ha-ras基因第12和61位密码子突变。
Mutations in the p53 gene are frequent genetic alterations in human hepatocellular carcinomas. We have examined 13 cases of human hepatocellular carcinomas from Germany for the presence of p53 aberrations in exons 4 to 8 of the gene by single-strand conformation polymorphism and restriction fragment-length polymorphism analyses and by sequencing of polymerase chain reaction products. Single base substitutions occurred in two human hepatocellular carcinomas: a C:G----T:A transition at a CpG site in codon 257, and a T:A----A:T transversion at codon 273. One of these point-mutated tumors and two additional tumors without point mutations demonstrated a loss of one p53 allele. None of the tumors was mutated in codons 12 or 61 of the c-Ha-ras gene.
影响因子：
11.2
作者：
A. Puisieux;Susan E. Lim;J. Groopman;M. Ozturk
通讯作者：
A. Puisieux;Susan E. Lim;J. Groopman;M. Ozturk
影响因子：
56.9
作者：
HOLLSTEIN, M;SIDRANSKY, D;HARRIS, CC
通讯作者：
HARRIS, CC
影响因子：
56.9
作者：
RIDEOUT, WM;COETZEE, GA;JONES, PA
通讯作者：
JONES, PA