A multiple-test study of anxiety-related behaviours in six inbred rat strains
A multiple-test study of anxiety-related behaviours in six inbred rat strains
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DOI：
10.1016/s0166-4328(96)00164-7
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发表时间：
1997-04-01
影响因子：
2.7
通讯作者：
Chaouloff, F
中科院分区：
文献类型：
--
作者：
Ramos, A;Berton, O;Chaouloff, F
最近的研究强调了遗传因素对焦虑的影响。在这种情况下，我们已经开始了一系列的实验，旨在选择，在六个近交系的大鼠，一对菌株，对比最大的恐惧相关的行为中测量的开放领域，高架十字迷宫，黑色和白色框和社会互动测试。所有行为指标均存在显著株间差异。所有变量的因素分析产生三个独立的因素解释总方差的85.1%。因子1具有来自与对厌恶刺激的接近/回避相关的变量的高负荷（例如，开放区域的中心、十字迷宫的开放臂和黑/白色盒的白色隔室）。与新环境中的一般活动相关的变量（例如，开放场中的总运动和十字迷宫中的闭臂进入）高度加载因子2。排便和社交时间对因子3有正向影响。为了验证因子1上增加的十字迷宫变量负荷是否与焦虑相关，在刘易斯和SHR大鼠中检查了地西泮和戊四唑单剂量的作用，即，关于因子1的最大对比菌株。变量与高负荷对这个因素的变化以相反的方式在地西泮和戊四唑治疗。因此，这项研究表明，刘易斯和SHR株可能构成一个有用的模型，用于研究焦虑基线水平个体间差异的神经生物学机制。
Recent studies have underlined the impact of genetic factors in anxiety profiles. In this context, we have initiated a series of experiments aiming to select, among six inbred strains of rats, a pair of strains that contrasts the most in fear-related behaviours measured in the open field, the elevated plus-maze, the black and white box and the social interaction test. Significant interstrain differences were found for all behavioural measures. A factor analysis of all variables produced three independent factors explaining 85.1% of the total variance. Factor 1 had high loadings from variables related to the approach/avoidance towards aversive stimuli (e.g., center of the open field, open arms of the plus-maze and white compartment of the black/white box). Variables related to general activity in novel environments (e.g., total locomotion in the open field and closed-arm entries in the plus-maze) loaded highly on Factor 2. Defecation and time of social interaction loaded positively on Factor 3. To verify whether elevated plus-maze variables loading on Factor 1 were associated to anxiety, the effects of single doses of diazepam and pentylenetetrazole were examined in Lewis and SHR rats, i.e., the most contrasting strains regarding Factor 1. Variables with high loadings on this factor changed in opposite ways in response to diazepam and pentylenetetrazole treatments. This study suggests, thus, that Lewis and SHR strains may constitute a useful model for studying the neurobiological mechanisms underlying the interindividual differences in baseline levels of anxiety.