Occurrence of TTX in a brackish water puffer "midorifugu", Tetraodon nigroviridis, collected from Thailand
Occurrence of TTX in a brackish water puffer "midorifugu", Tetraodon nigroviridis, collected from Thailand
复制标题
DOI：
10.3358/shokueishi.40.5_363
复制
发表时间：
1999-10-01
期刊：
影响因子：
--
通讯作者：
Noguchi, T
中科院分区：
文献类型：
--
作者：
Mahmud, Y;Yamamori, K;Noguchi, T
对1996年11月至1998年5月从泰国采集的咸水河鲀（Tetraodon nigroviridis）的毒性进行了筛选试验。在不同组织中，皮肤的毒性得分最高，范围为49.2-286 MU/g，其次是肌肉（5.4-11 MU/g），而肝脏和肠道的得分大多低于5 MU/g。采用活性炭、Bio-Gel P-2和Bio-Rex 70三种柱层析对毒素进行部分纯化。经HPLC、TLC、电泳、核磁共振氢谱和液质联用分析，鉴定纯化后的毒素为河豚毒素。
Screening tests were carried out on the toxicity of a brackish water puffer, Tetraodon nigroviridis, collected from Thailand through November, 1996 to May, 1998. Among the different tissues, the skin showed the highest toxicity scores, ranging from 49.2-286 MU/g, followed by muscle (5.4-11 MU/g), while liver and intestine mostly gave scores under 5 MU/g. The toxin was partially purified by three kinds of column chromatography consisting of activated charcoal, Bio-Gel P-2 and Bio-Rex 70. Analyses by HPLC, TLC, electrophoresis,H-1-NMR and LC/MS identified the purified toxin as tetrodotoxin.