Downregulation of P2X7 receptor expression in rat oligodendrocyte precursor cells after hypoxia ischemia
Downregulation of P2X7 receptor expression in rat oligodendrocyte precursor cells after hypoxia ischemia
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DOI：
10.1002/glia.20758
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发表时间：
2009-02
期刊：
影响因子：
6.2
通讯作者：
Li-yan Wang;W. Cai;Peng-hui Chen;Qi-yue Deng;Cong Zhao
中科院分区：
文献类型：
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作者：
Li-yan Wang;W. Cai;Peng-hui Chen;Qi-yue Deng;Cong Zhao
Oligodendrocyte precursor cells (OPCs) are the predominant oligodendrocyte‐lineage stage in the cerebral hemispheres of neonatal rat. Prior studies have shown that OPCs are highly vulnerable to hypoxic‐ischemic injury, yet the mechanisms are not well understood. P2X7 receptor (P2X7R) is an ATP‐gated ion channel that has unusual properties and plays very complex roles in a variety of neuropathologic conditions. However, little is known about the involvement of P2X7R in OPCs development and injury. The present study was aimed at examining the presence of P2X7R in OPCs and evaluating the change of the receptor expression after hypoxia ischemia. Using Immunofluorescence, RT‐PCR, and western blot analysis, we demonstrated that OPCs expressed P2X7R in vitro and in vivo. Activation of P2X7R in OPCs in response to 3′‐O‐(4‐benzoyl) benzoyl‐ATP (BzATP) led to an increased mobilization of intracellular calcium [Ca2+]i, formation of large pores and cell death. These functional responses were sensitive to pretreatment of cells with the P2X7R antagonist, Brilliant Blue G (BBG, 100 nM), which was a selective antagonist for P2X7R in nanomole range. A decrease in P2X7R expression was observed in cultured OPCs after exposure to oxygen‐glucose deprivation (OGD) for 2 h in vitro. Using a neonatal hypoxic‐ischemic injury model in postnatal 3 rats, the similar downregulation was also detected in ischemic cerebral cortex, subcortical white matter and hippocampus compared with sham operation controls. In conclusion, the present data demonstrated that OPCs expressed functional P2X7R. The post‐ischemic downregulation of P2X7R suggested a role for this receptor in the pathophysiology of hypoxic‐ischemic brain injury. © 2008 Wiley‐Liss, Inc.
