Pump-Free Microfluidic Rapid Mixer Combined with a Paper-Based Channel
Pump-Free Microfluidic Rapid Mixer Combined with a Paper-Based Channel
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DOI：
10.1021/acssensors.0c00937
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发表时间：
2020-07-24
期刊：
影响因子：
8.9
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
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作者：
Jang, Ilhoon;Carrao, Daniel B.;Henry, Charles S.
毛细管力通常用于在无泵微流体平台（例如纸基微流体）中传输流体。然而，由于纸是一种由不均匀纤维素纤维组成的多孔材料，因此在执行混合等稳定流动功能方面存在一些局限性。在这里，我们开发了一种无泵微流体装置，可以通过结合纸和塑料来实现快速混合。该装置是通过层压透明薄膜和双面胶制成的，由一个末端位于纸基反应区域的重叠入口组成。使用水性染料和 pH 指示剂通过实验证实了所开发装置的混合性能。此外，通过数值计算微流体通道上的浓度场来评估绝对混合指数。为了证明新方法的实用性，使用比色酶抑制测定法对有机磷农药进行了检测。开发的设备和智能手机应用程序用于检测食品样品中的有机磷农药，展示了现场分析的潜力。
Capillary forces are commonly employed to transport fluids in pump-free microfluidic platforms such as paper-based microfluidics. However, since paper is a porous material consisting of nonuniform cellulose fibers, it has some limitations in performing stable flow functions like mixing. Here, we developed a pump-free microfluidic device that enables rapid mixing by combining paper and plastic. The device was fabricated by laminating transparency film and double-sided adhesive and is composed of an overlapping inlet ending in a paper-based reaction area. The mixing performance of the developed device was confirmed experimentally using aqueous dyes and pH indicators. In addition, the absolute mixing index was evaluated by numerically calculating the concentration field across the microfluidic channels. To demonstrate the utility of the new approach, the detection of an organophosphate pesticide was carried out using a colorimetric enzymatic inhibition assay. The developed device and a smartphone application were used to detect organophosphate pesticide on food samples, demonstrating the potential for onsite analysis.