Chloride channels in the nuclear membrane
Chloride channels in the nuclear membrane
复制标题
核膜中的氯离子通道
DOI：
10.1007/bf01993962
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
D. Clapham
中科院分区：
文献类型：
--
作者：
L. Tabares;M. Mazzanti;D. Clapham
从离体大鼠肝细胞核测定氯离子选择性通道。单离子通道电流记录在“核连接”和切除的补丁在insideout配置的膜片钳技术。两种类型的氯离子电导被定义，一个大的电导（150 pS;iCl.N）通道与复杂的动力学和多个substates，和第二个较小的电导（58 pS;ICl.N）通道敏感的阻断ATP。通道被已知阻断氯离子通道的药物抑制，并且对钙和镁的内部和外部变化不敏感。据推测，这些通道位于核双膜的外膜中，并可能介导从核周空间释放和摄取钙的电荷平衡。
SummaryChloride-selective ion channels were measured from isolated rat liver nuclei. Single ion channel currents were recorded in both “nuclear-attached” and in excised patches in the insideout configuration of the patch-clamp technique. Two types of chloride conductance were defined, a large conductance (150 pS;iCl.N) channel with complex kinetics and multiple substates, and a second smaller conductance (58 pS;ICl.n) channel sensitive to block by ATP. The channels were inhibited by pharmacological agents known to block chloride channels and were insensitive to internal and external changes in calcium and magnesium. Presumably the channels reside in the external membrane of the nuclear double membrane and may mediate charge balance in the release and uptake of calcium from the perinuclear space.
影响因子：
3.6
作者：
M. M. White-M.;M. Aylwin
通讯作者：
M. M. White-M.;M. Aylwin