The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
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GATA转录因子GNC在杨树的光合作用和生长中发挥着重要作用
DOI：
10.1093/jxb/erz564
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发表时间：
2020-03-25
影响因子：
6.9
通讯作者：
Xia, Xinli
中科院分区：
文献类型：
--
作者：
An, Yi;Zhou, Yangyan;Xia, Xinli
加塔转录因子参与拟南芥和水稻不同生长过程和环境响应的调控。本研究对木本多年生大果杨中的加塔家族进行了全面的生物信息学调查。根据基因结构和系统发育关系将39个杨树加塔GATA基因分为4个亚科。预测的顺式元件表明，白杨加塔基因在光，植物激素，发育和胁迫反应的潜在作用。从速生白杨无性系中克隆到一个与加塔硝酸盐诱导的碳代谢有关的白杨加塔基因PdGATA 19/PdGNC。PdGNC的表达显着上调，在高（50 mM）和低（0.2 mM）硝酸盐浓度下的叶片。CRISPR/Cas9介导的突变体crispr-GNC表现出严重的生长迟缓和次生木质部分化增强。与野生型相比，PdGNC过表达转化体表现出25-30%的生长速度，20-28%的生物量积累，以及类似于25%的叶绿素含量，光合速率和株高的增加。转录组学分析表明，PdGNC参与了叶片光合电子传递和碳同化、茎细胞分裂和碳水化合物利用以及根的氮吸收。这些数据表明PdGNC在植物生长中起着至关重要的作用，并且在树木分子育种中具有潜在的用途。
GATA transcription factors are involved in the regulation of diverse growth processes and environmental responses in Arabidopsis and rice. In this study, we conducted a comprehensive bioinformatic survey of the GATA family in the woody perennial Populus trichocarpa. Thirty-nine Populus GATA genes were classified into four subfamilies based on gene structure and phylogenetic relationships. Predicted cis-elements suggested potential roles of poplar GATA genes in light, phytohormone, development, and stress responses. A poplar GATA gene, PdGATA19/PdGNC (GATA nitrate-inducible carbon-metabolism-involved), was identified from a fast growing poplar clone. PdGNC expression was significantly up-regulated in leaves under both high (50 mM) and low (0.2 mM) nitrate concentrations. The CRISPR/Cas9-mediated mutant crispr-GNC showed severely retarded growth and enhanced secondary xylem differentiation. PdGNC-overexpressing transformants exhibited 25-30% faster growth, 20-28% higher biomass accumulation, and similar to 25% increase in chlorophyll content, photosynthetic rate, and plant height, compared with the wild type. Transcriptomic analysis showed that PdGNC was involved in photosynthetic electron transfer and carbon assimilation in the leaf, cell division and carbohydrate utilization in the stem, and nitrogen uptake in the root. These data indicated that PdGNC plays a crucial role in plant growth and is potentially useful in tree molecular breeding.