Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited
Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited
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DOI：
10.1016/s1097-2765(02)00558-0
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发表时间：
2002-06-01
期刊：
影响因子：
16
通讯作者：
Strouboulis, J
中科院分区：
文献类型：
--
作者：
de Krom, M;van de Corput, M;Strouboulis, J
我们已经进行了详细的表征小鼠珠蛋白基因表达模式的细胞核和细胞质中的单个红细胞。我们证明了α-与β-珠蛋白表达的不平衡，在一个显着的比例细胞核转录模式和细胞质mRNA水平。克隆细胞分析表明，这些表达模式是克隆遗传的，而多拷贝转基因位点的分析表明，在一个位点的所有基因的激活全或无效果。这些数据提供了强有力的证据，珠蛋白基因激活的随机基础，导致遗传的全或无表达模式。
We have undertaken a detailed characterization of mouse globin gene expression patterns in the nucleus and cytoplasm of single erythroid cells. We demonstrate an imbalance of alpha- versus beta-globin expression in a significant proportion of cells both in nuclear transcription patterns and cytoplasmic mRNA levels. Clonal cell analysis showed these expression patterns to be clonally inherited, while analysis of a multicopy transgenic locus showed an all-or-none effect in the activation of all the genes in one locus. These data provide strong evidence for a stochastic basis of globin gene activation resulting in heritable all-or-none expression patterns.