A recombinant chimeric antigen toward a standardized serodiagnosis of Taenia solium neurocysticercosis
A recombinant chimeric antigen toward a standardized serodiagnosis of Taenia solium neurocysticercosis
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DOI：
10.1002/prca.200800084
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发表时间：
2008-12-01
影响因子：
2
通讯作者：
Kong, Yoon
中科院分区：
文献类型：
--
作者：
Bae, Young-An;Jeong, Yeon-Tack;Kong, Yoon
脑囊虫病（NC）在世界范围内引起了严重的神经系统问题。以往的蛋白质组学分析表明，猪带绦虫囊液中的低分子量蛋白可能主要来源于120 kDa（由14-38 kDa亚基组成）和150 kDa（7-15 kDa亚基组成）两个大分子。我们表征了这两种蛋白的血清学特性，并建立了一种免疫嵌合体。120和150 kDa蛋白质具有粗CF的54-81和94-98%的抗体结合活性，彼此具有最小的抗原交叉反应性。150 kDa亚基的表达和免疫识别相对恒定，无论收集的CF的不同地理来源如何，而120 kDa亚基的表达和免疫识别因其来源而异（亚洲与美洲）。我们克隆了四个代表性的蛋白质（一个来自120 kDa和三个来自150 kDa），显示不同的表位特异性，产生嵌合体，并证明这种方案可以支持血清学诊断的可靠性。针对活动期/混合期NC和其他感染以及健康对照血清的总体灵敏度和特异性分别为97.5%（156/160份样本）和97.8%（265/271例病例）。成人带绦虫病、裂头蚴病和片形吸虫病患者血清显示弱交叉反应。Micro-ELISA显示类似的结果。该嵌合体可用于NC血清学诊断标准化平台的构建。
Neurocysticercosis (NC) invokes formidable neurological problems worldwide. Previous proteomic analyses revealed most of the low-molecular-weight proteins might derive from two macromolecules of 120 kDa (consisting of 14-38 kDa subunits) and 150 kDa (7-15 kDa subunits) of Taenia solium metacestode (TsM) cyst fluid (CF). We characterized serological properties of these two proteins and established an inimunopotent chimera. The 120 and 150 kDa proteins harbored 54-81 and 94-98% of the antibody-binding activity of the crude CF with minimal antigenic cross-reactivity to each other. The expression and immune recognition of the 150 kDa subunits were relatively constant, regardless of the different geographical origins of the CF collected, while those of the 120 kDa subunits varied by their origins (Asia vs. America). We cloned four representative proteins (one from the 120 kDa and three from the 150 kDa) that showed different epitope specificities, generated a chimera, and demonstrated that this regimen may bolster serodiagnostic reliability. Overall sensitivity and specificity, against sera from active-/mixed-stage NC and those from other infections, and healthy-controls, were determined to be 97.5% (156/160 samples) and 97.8% (265/271 cases). Patient sera from adult taeniases, sparganosis, and fascioliasis showed weak cross-reactions. Micro-ELISA showed similar results. This chimera may prove useful in the construction of standardized platform for NC serodiagnosis.