Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates
Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates
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DOI：
10.1126/science.1078068
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发表时间：
2003-01-03
期刊：
影响因子：
56.9
通讯作者：
Wu, C
中科院分区：
文献类型：
--
作者：
Shen, XT;Xiao, H;Wu, C
真核生物使用三磷酸腺苷（ATP）依赖的染色质重塑复合物来调节基因表达。在这里，我们表明，肌醇多磷酸可以调节几个染色质重塑复合物在体外的活动。肌醇六磷酸（IP 6）抑制核小体动员的BMPF，ISW 2，和INO 80复合物。相比之下，核小体动员酵母SWI/SNF复合物刺激肌醇四磷酸（IP 4）和肌醇五磷酸（IP 5）。我们证明，在基因编码肌醇多磷酸激酶，产生IP 4，IP 5和IP 6的突变损害体内转录。这些结果提供了肌醇多磷酸，染色质重塑和基因表达之间的联系。
Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin-remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakisphosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.