ULTRASTRUCTURAL STUDIES ON A MUTANT OF BACILLUS-SUBTILIS WHOSE GROWTH IS INHIBITED DUE TO INSUFFICIENT AUTOLYSIN PRODUCTION
ULTRASTRUCTURAL STUDIES ON A MUTANT OF BACILLUS-SUBTILIS WHOSE GROWTH IS INHIBITED DUE TO INSUFFICIENT AUTOLYSIN PRODUCTION
复制标题
DOI：
10.1128/jb.109.3.1247-1257.1972
复制
发表时间：
1972-01-01
影响因子：
3.2
通讯作者：
CUNNINGHAM, WP
中科院分区：
文献类型：
--
作者：
FAN, DP;BECKMAN, MM;CUNNINGHAM, WP
枯草芽孢杆菌βA177的生长在特定的条件下受到抑制，即不能产生足够的自溶酶来达到最佳生长。电子显微镜研究表明，在生长抑制期间，在细胞弯曲的位置处存在细胞壁的局部增厚。提出了一个模型来解释这一结果。通过添加溶菌酶或aB可以恢复快速生长。将枯草杆菌自溶素混合物添加至生长抑制的βA177培养物。这种添加减少了局部壁增厚，并导致其他变化的表面形态，这是描述和讨论。当裂解酶水平降低时，隔膜形成似乎比细胞伸长受到的抑制相对较小。测量结果表明，在51 ℃下生长的βA177培养物中，细胞端部的细胞壁与细胞侧面的细胞壁在形态上不同。
The growth ofBacillus subtilismutant βA177 can be inhibited under special conditions in which not enough autolytic enzymes are produced for optimal growth. Electron microscopy studies show that during growth inhibition there is localized thickening of the cell wall at positions where cells bend. A model is proposed to explain this result. Rapid growth can be restored by adding lysozyme or aB. subtilisautolysin mixture to a growth-inhibited βA177 culture. Such addition reduces the localized wall thickening and causes other changes in surface morphology which are described and discussed. Septum formation seems to be relatively less inhibited than cell elongation when lytic enzyme levels are reduced. Measurements were made demonstrating that walls at ends of cells are morphologically different from walls at sides of cells in cultures of βA177 growing at 51 C.