Volatile profiles of fungi - Chemotyping of species and ecological functions
Volatile profiles of fungi - Chemotyping of species and ecological functions
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DOI：
10.1016/j.fgb.2013.02.005
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发表时间：
2013-05-01
影响因子：
3
通讯作者：
Rosenkranz, Maaria
中科院分区：
文献类型：
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作者：
Mueller, Anna;Faubert, Patrick;Rosenkranz, Maaria
真菌释放大量挥发性有机化合物 (VOC)。在本研究中，我们对外生菌根 (EM)、致病性和腐生真菌物种的气味特征进行了表征和比较，旨在将这些模式用作化学分型工具。使用搅拌棒吸附萃取法从八种真菌物种（包括九个菌株（四种EM、三种病原体和两种腐生菌））的顶部空间收集挥发物，并通过气相色谱-质谱法（GC-MS）进行分析。去除生长系统释放的 VOC 后，检测到 54 种 VOC，其中包括 15 种以前在真菌中未报道过的新型化合物。主成分和聚类分析表明，真菌物种的气味特征不同，特别是倍半萜烯的模式。官能团和物种可以通过使用其特定的发射模式进行化学分型。不同生态群体的预测概率为 90-99%，而对于单个物种，预测概率在 55% 到 83% 之间。这项研究强烈支持挥发性化合物的分析可用于不同功能真菌群的非侵入性鉴定的概念。 (C) 2013 Elsevier Inc. 保留所有权利。
Fungi emit a large spectrum of volatile organic compounds (VOCs). In the present study, we characterized and compared the odor profiles of ectomycorrhizal (EM), pathogenic and saprophytic fungal species with the aim to use these patterns as a chemotyping tool. Volatiles were collected from the headspace of eight fungal species including nine strains (four EM, three pathogens and two saprophytes) using the stir bar sorptive extraction method and analyzed by gas chromatography - mass spectrometry (GC-MS). After removal of VOCs released from the growth system, 54 VOCs were detected including 15 novel compounds not reported in fungi before. Principle component and cluster analyses revealed that fungal species differ in their odor profiles, particularly in the pattern of sesquiterpenes. The functional groups and species could be chemotyped by using their specific emission patterns. The different ecological groups could be predicted with probabilities of 90-99%, whereas for the individual species the probabilities varied between 55% and 83%. This study strongly supports the concept that the profiling of volatile compounds can be used for non-invasive identification of different functional fungal groups. (C) 2013 Elsevier Inc. All rights reserved.