Comparison of the Brown Sugar, Hot Water, and Salt Methods for Detecting Western Cherry Fruit Fly (Diptera: Tephritidae) Larvae in Sweet Cherry
Comparison of the Brown Sugar, Hot Water, and Salt Methods for Detecting Western Cherry Fruit Fly (Diptera: Tephritidae) Larvae in Sweet Cherry
复制标题
红糖、热水和盐法检测甜樱桃中西樱桃实蝇（双翅目：实蝇科）幼虫的比较
DOI：
10.1653/024.097.0212
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发表时间：
2014
影响因子：
1.9
通讯作者：
W. Yee
中科院分区：
文献类型：
--
作者：
W. Yee
摘要
为保证检疫安全，建立了采后检测实蝇幼虫的红糖法和热水法。确定这些方法的修改是否可以产生更好的结果以及是否存在更便宜的替代方法将是有益的。本文报道了从甜樱桃（Prunus avium [L.] L.）的范围内。通过红糖浮选与热水和氯化钠（盐）浮选方法的比较。用标准的樱桃压碎机压碎和切碎樱桃，并浸没在红糖溶液、热水或盐溶液中。在糖和盐溶液中，提取的幼虫漂浮，而在热水中，它们下沉;并在所有3个幼虫进行目视检查。红糖浮选法检测到更多的总幼虫比热水的方法时，使用一个明确的盘子或黑色的平底锅后，樱桃被粉碎通过一个2毫米的差距，在樱桃破碎机，分别导致95，85和85%的检测。红糖和盐浮选方法导致类似的检测率85-99%后，樱桃通过2毫米或5毫米的间隙切碎，即使2毫米的间隙导致更大的切碎。在红糖、热水和盐溶液中，检测到26-88%的第一龄幼虫（当至少有8个个体时），而第二和第三龄幼虫的检测率为77-100%。结果表明，盐和红糖溶液同样有效地检测R。从压碎的樱桃中分离出来的冷漠。盐溶液比红糖溶液更有利，因为它更便宜。如果盐溶液用于检测樱桃中的幼虫斑点翅果蝇（Drosophila suzukii [Matsumura]），目前的结果表明，它不会影响R。冷漠
Abstract
Brown sugar and hot water methods have been developed to detect larvae of tephritid fruit flies post-harvest in fruit in order to maintain quarantine security. It would be useful to determine if modifications of these methods can yield better results and if less expensive alternatives exist. This study reports detection rates of Rhagoletis indifferens Curran (Diptera: Tephritidae) larvae separated from crushed sweet cherries (Prunus avium [L.] L.) by brown sugar flotation versus hot water and sodium chloride (salt) flotation methods. Cherries were crushed and shredded by a standard cherry crusher and submerged either in brown sugar solution, hot water, or salt solution. In sugar and salt solutions, extracted larvae floated, whereas in hot water they sank; and in all 3 visual inspections for larvae were made. The brown sugar flotation method detected more total larvae than the hot water method when using a clear dish or black pan after cherries were shredded through a 2-mm gap in a cherry crusher, resulting in 95, 85, and 85% detections, respectively. Brown sugar and salt flotation methods resulted in similar detection rates of 85–99% after cherries were shredded through either a 2- or 5-mm gap, even though the 2-mm gap resulted in greater shredding. In brown sugar, hot water, and salt solutions, 26–88% of first instars (when there were at least 8 individuals) were detected versus 77–100% of second and third instars. Results demonstrate that salt and brown sugar solutions are equally efficacious for detecting larvae of R. indifferens separated from crushed cherries. Salt solution is advantageous over brown sugar solution because it is less expensive. Should salt solution be used for detecting larval spotted wing drosophila (Drosophila suzukii [Matsumura]) in cherries, current results show that it would not compromise detection of R. indifferens.