Genetic Characterization of Venezuelan Equine Encephalitis Virus from Bolivia, Ecuador and Peru: Identification of a New Subtype ID Lineage
Genetic Characterization of Venezuelan Equine Encephalitis Virus from Bolivia, Ecuador and Peru: Identification of a New Subtype ID Lineage
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DOI：
10.1371/journal.pntd.0000514
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发表时间：
2009-09-01
影响因子：
3.8
通讯作者：
Kochel, Tadeusz J.
中科院分区：
文献类型：
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作者：
Aguilar, Patricia V.;Adams, A. Paige;Kochel, Tadeusz J.
委内瑞拉马脑炎病毒（VEEV）是美洲数十万人和马严重疾病病例的原因。在秘鲁、玻利维亚和厄瓜多尔进行了一项被动监测研究，以确定发热性疾病的虫媒病毒病因。将患有持续时间< 7天的疑似病毒相关的急性未分化发热性疾病的患者纳入研究，并从每位患者获得血液样品并通过病毒分离进行测定。在自愿入组时还获得了每例患者的人口统计学和临床信息。在2005 - 2007年，委内瑞拉马脑炎（VEE）的情况下，第一次被诊断为居民的玻利维亚，病人没有报告旅行，这表明地方性流通的VEEV在玻利维亚。2001年和2003年，厄瓜多尔也发现了VEE病例。自1993年以来，VEEV一直从秘鲁的洛雷托患者中分离，最近（2005年）在秘鲁的马德雷德迪奥斯分离。我们对来自玻利维亚、厄瓜多尔和秘鲁的VEEV进行了系统发育分析，并将它们与来自南美洲其他地区的菌株的关系进行了比较。我们发现VEEV亚型ID巴拿马/秘鲁基因型是在秘鲁流行的主要基因型。我们还证明了在厄瓜多尔流行的VEEV亚型ID株属于哥伦比亚/委内瑞拉基因型，而来自秘鲁的马德雷德迪奥斯和玻利维亚的科恰班巴的VEEV属于一种新的ID基因型。总之，我们确定了一个新的主要谱系的地方性VEEV亚型ID，信息，可以帮助理解的出现和进化的VEEV在南美洲。
Venezuelan equine encephalitis virus (VEEV) has been responsible for hundreds of thousands of human and equine cases of severe disease in the Americas. A passive surveillance study was conducted in Peru, Bolivia and Ecuador to determine the arboviral etiology of febrile illness. Patients with suspected viral-associated, acute, undifferentiated febrile illness of < 7 days duration were enrolled in the study and blood samples were obtained from each patient and assayed by virus isolation. Demographic and clinical information from each patient was also obtained at the time of voluntary enrollment. In 2005 2007, cases of Venezuelan equine encephalitis (VEE) were diagnosed for the first time in residents of Bolivia; the patients did not report traveling, suggesting endemic circulation of VEEV in Bolivia. In 2001 and 2003, VEE cases were also identified in Ecuador. Since 1993, VEEV has been continuously isolated from patients in Loreto, Peru, and more recently (2005), in Madre de Dios, Peru. We performed phylogenetic analyses with VEEV from Bolivia, Ecuador and Peru and compared their relationships to strains from other parts of South America. We found that VEEV subtype ID Panama/Peru genotype is the predominant one circulating in Peru. We also demonstrated that VEEV subtype ID strains circulating in Ecuador belong to the Colombia/Venezuela genotype and VEEV from Madre de Dios, Peru and Cochabamba, Bolivia belong to a new ID genotype. In summary, we identified a new major lineage of enzootic VEEV subtype ID, information that could aid in the understanding of the emergence and evolution of VEEV in South America.