Interleukin-6 family cytokine M17 induces differentiation and nitric oxide response of goldfish (Carassius auratus L.) macrophages
Interleukin-6 family cytokine M17 induces differentiation and nitric oxide response of goldfish (Carassius auratus L.) macrophages
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DOI：
10.1016/j.dci.2006.12.001
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Belosevic, Miodrag
中科院分区：
文献类型：
--
作者：
Hanington, Patrick C.;Belosevic, Miodrag
属于白细胞介素-6（IL-6）家族的细胞因子在许多生物学过程中起作用，并且在该家族内显示出高度冗余。虽然已经鉴定了许多来自硬骨鱼的类似于IL-6家族细胞因子的mRNA转录物，但是没有一个被功能性地表征以确认它们的身份。我们报告的识别和功能特性的金鱼M17，具有类似的哺乳动物和鸟类睫状神经营养因子和哺乳动物白血病抑制因子的序列和mRNA表达模式的分子。发现金鱼M17在与细胞条件培养基组合添加时诱导金鱼巨噬细胞中一氧化氮的产生并刺激巨噬细胞祖细胞的增殖。此外，发现金鱼M17在生物学相关浓度下促进分选的单核细胞分化为巨噬细胞。(c)2007爱思唯尔有限公司，保留所有权利。
Cytokines belonging to the interleukin-6 (IL-6) family function in many biological processes and display a high degree of redundancy within the family. Although a number of mRNA transcripts from teleost fish that resemble IL-6 family cytokines have been identified none have been functionally characterized to confirm their identity. We report on the identification and functional characterization of goldfish M17, a molecule possessing sequence and mRNA expression patterns similar to the mammalian and avian ciliary neurotrophic factor and mammalian leukemia inhibitory factor. Goldfish M17 was found to induce the production of nitric oxide in goldfish macrophages and stimulate the proliferation of macrophage progenitor cells when added in combination with cell-conditioned medium. Moreover, goldfish M17 was found to facilitate the differentiation of sorted monocytes into macrophages at biologically relevant concentrations. (c) 2007 Elsevier Ltd, All rights reserved.