Complete mitochondrial genome of the grass carp (Ctenopharyngodon idella, Teleostei): Insight into its phylogenic position within Cyprinidae
Complete mitochondrial genome of the grass carp (Ctenopharyngodon idella, Teleostei): Insight into its phylogenic position within Cyprinidae
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DOI：
10.1016/j.gene.2008.07.011
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发表时间：
2008-11-15
期刊：
影响因子：
3.5
通讯作者：
Li, Sifa
中科院分区：
文献类型：
--
作者：
Wang, Chenghui;Chen, Qin;Li, Sifa
基因组比较在基础研究和应用研究的许多领域都有重要的意义，包括物种的遗传学研究。草鱼（Ctenopharyngodon idella）属于鲤科（Cyprinidae），是世界上最大的淡水鱼类，但其隶属于哪个亚科一直是一个有争议的问题。本研究测定了草鱼线粒体基因组全序列，并对所有线粒体蛋白编码基因和一个核基因（RAG 2）进行了系统发育分析，以探讨草鱼与其他鲤科鱼类的进化关系。草鱼有丝分裂基因组全长16，609 bp。与大多数其他脊椎动物一样，它包含相同的基因顺序和相同数量的基因或区域，包括13个蛋白质编码基因，2个rRNA基因，22个tRNA基因和一个推定的控制区。使用两种不同的数据集（线粒体和核）和三种不同的计算算法（贝叶斯，MP和ML）的系统发育分析都显示了两个不同的群体具有较高的统计支持，表明鲤亚科和雅罗鱼亚科是两个独立的，有效的亚科。重要的是，我们的系统发育结果提供了强有力的分子证据，支持放置在银汉鱼父亲比鲤科草鱼。(c)2008 Elsevier B.V.版权所有
Genome comparison has shed light on many fields of both basic and applied research, including the study of species phylogeny. Grass carp (Ctenopharyngodon idella) belongs to Cyprinidae, the largest freshwater fish family; but which subfamily it belongs to remains a controversial issue. In this study, the complete mitochondrial genome (mitogenome) sequence of grass carp was determined and phylogenetic analyses of all mitochondrial protein-coding genes and a nuclear gene (RAG 2) were conducted to explore the evolutionary relationship of grass carp with other cyprinid species. The mitogenome of grass carp is 16,609 bp in length. As with most other vertebrates, it contains the same gene order and an identical number of genes or regions, including 13 protein-coding genes, two rRNA genes, 22 tRNA genes and one putative control region. Phylogenetic analyses using two different datasets (mitochondrial and nuclear) and three different computational algorithms (Bayesian, MP and ML) all revealed two distinct groups with high statistical support, indicating that Cyprininae and Leuciscinae are two separate, valid subfamilies. Importantly, our phylogenetic result provides strong molecular evidence in support of the placement of Ctenopharyngodon in Leuciscinae Father than in Cyprininae. (c) 2008 Elsevier B.V. All rights reserved