Echinococcus multilocularis primary cells: Improved isolation, small-scale cultivation and RNA interference
Echinococcus multilocularis primary cells: Improved isolation, small-scale cultivation and RNA interference
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DOI：
10.1016/j.molbiopara.2010.07.001
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发表时间：
2010-11-01
影响因子：
1.5
通讯作者：
Gottstein, Bruno
中科院分区：
文献类型：
--
作者：
Spiliotis, Markus;Mizukami, Chiaki;Gottstein, Bruno
在这项研究中，我们在转录和翻译水平上证明了RNA干扰介导的多房棘球蚴原代细胞中靶基因表达的敲除。此外，我们还报道了一种从体外培养的囊泡中产生多房棘原代细胞微聚集体的改进方法，以及在体外长时间培养少量转染小干扰rna的细胞的方法。这可以评估RNA干扰对棘球蚴原代细胞的影响，包括体外生长、增殖、寄生虫的分化和新型囊泡的形成。(C) 2010 Elsevier B.V.版权所有
In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro. (C) 2010 Elsevier B.V. All rights reserved.