Anti-hypoxic activity of the ethanol extract from Portulaca oleracea in mice
Anti-hypoxic activity of the ethanol extract from Portulaca oleracea in mice
复制标题
马齿苋乙醇提取物对小鼠的抗缺氧活性。
DOI：
10.1016/j.jep.2009.04.028
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发表时间：
2009-07-15
影响因子：
5.4
通讯作者：
Li, Min
中科院分区：
文献类型：
--
作者：
Chen, Cheng-Jie;Wang, Wan-Yin;Li, Min
研究目的：研究马齿苋乙醇提取物（EEPO）对小鼠缺氧模型的影响，探讨其抗缺氧作用的可能机制，为马齿苋的抗缺氧作用提供科学依据。在几种缺氧小鼠模型中评价EEPO的抗缺氧活性，包括密闭常压缺氧和亚硝酸钠或氰化钾中毒。结果：EEPO 100、200、400 mg/kg灌胃给药，能剂量依赖性地延长常压和化学性缺氧小鼠的存活时间，并能显著延长缺氧小鼠的存活时间。糖酵解酶活性和ATP水平均高于对照组。在戊巴比妥钠诱导的睡眠时间试验和旷场试验中，EEPO既不显著增加戊巴比妥钠诱导的睡眠时间，也不显著损害运动性能，表明所观察到的抗缺氧活性不太可能是由于镇静或运动异常。结果表明，EEPO具有显著的抗缺氧活性，这可能与促进糖酵解关键酶的活性和提高缺氧小鼠体内ATP水平有关。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Aim of the study: To investigate the effects of the ethanol extract from Portulaca oleracea (EEPO) on hypoxia models mice and to find the possible mechanism of its anti-hypoxic actions so as to elucidate the anti-hypoxia activity and provide scientific basis for the clinical use of Portulaca oleracea.Materials and methods: EEPO was evaluated on anti-hypoxic activity in several hypoxia mice models, including closed normobaric hypoxia and sodium nitrite or potassium cyanide toxicosis. To verify the possible mechanism(s), we detected the activities of pyruvate kinase (PK), phosphofructokinase (PFK), lactate dehydrogenase (LDH) and the level of adenosine triphosphate (ATP) in mice cortices.Results: Given orally, the EEPO at doses of 100, 200, 400 mg/kg could dose-dependently enhance the survival time of mice in both of the normobaric and chemical hypoxia models. The activity of the glycolysis enzymes and the level of ATP were higher than those of the control. In the pentobarbital sodium-induced sleeping time test and the open-field test, EEPO neither significantly enhanced the pentobarbital sodium-induced sleeping time nor impaired the motor performance, indicating that the observed anti-hypoxic activity was unlikely due to sedation or motor abnormality.Conclusions: These results demonstrated that the EEPO possessed notable anti-hypoxic activity, which might be related to promoting the activity of the key enzymes in glycolysis and improving the level of ATP in hypoxic mice. (C) 2009 Elsevier Ireland Ltd. All rights reserved.