Development of a molecular marker to identify a candidate line of turmeric (Curcuma longa L.) with a high curcumin content.
Development of a molecular marker to identify a candidate line of turmeric (Curcuma longa L.) with a high curcumin content.
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DOI：
10.4236/ajps.2011.21002
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Yamamoto, Y.
中科院分区：
文献类型：
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作者：
Hayakawa, H.;Kobayashi, T.;Yamamoto, Y.
以姜黄（Curcuma longa L.）姜黄素在传统医学中是众所周知的，并且姜黄素广泛用于各种地理区域。虽然在该物种内姜黄素的量存在差异，但由于其形态特征相对简单，难以通过根茎识别候选系。准确识别C.姜黄素含量高，我们分析了几个叶绿体DNA序列。首先，确定合适的外群分类群进行种下分析的C。longa，构建了C.龙属及其近缘种。结果表明，C. aromatica和C. zedoaria与C. longa其次，建立了一个用于鉴定C.姜黄素含量高的龙眼，利用叶绿体微卫星区域进行网络分析。结果表明，在C. Longa对应于高姜黄素含量线。因此，用于分析的叶绿体微卫星区域使我们能够确定具有高姜黄素含量的该物种的品系。
Dried and fresh rhizomes of the spice turmeric (Curcuma longa L.) are well known in traditional medicine, and curcumin is widely used in various geographic regions. Although there are differences in the amount of curcumin within this species, identification of the candidate line by rhizome is difficult because of the relative simplicity of its morphological characteristics. To accurately identify lines of C. longa with a high content of curcumin, we analysed several sequences of chloroplast DNA. First, to determine the appropriate outgroup taxa in which to conduct infraspecific analyses of C. longa, we reconstructed the molecular phylogenetic tree of C. longa and its allied species. The results showed that C. aromatica and C. zedoaria are closely related to C. longa. Next, to develop a molecular marker for identifying lines of C. longa with a high content of curcumin, a network analysis using chloroplast microsatellite regions was performed. Results showed that a unique haplotype within C. longa corresponds to the high curcumin content line. Therefore, the chloroplast microsatellite regions used for the analysis allowed us to determine the lines of this species with high curcumin content.