Overexpression of UDP-glucose pyrophosphorylase from Larix gmelinii enhances vegetative growth in transgenic Arabidopsis thaliana
Overexpression of UDP-glucose pyrophosphorylase from Larix gmelinii enhances vegetative growth in transgenic Arabidopsis thaliana
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DOI：
10.1007/s00299-013-1558-3
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发表时间：
2014-01
影响因子：
6.2
通讯作者：
Ningning Li;Li Wang;Wenbo Zhang;K. Takechi;H. Takano;Xiaofei Lin
中科院分区：
文献类型：
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作者：
Ningning Li;Li Wang;Wenbo Zhang;K. Takechi;H. Takano;Xiaofei Lin
从落叶松中克隆到一个UDP-葡萄糖焦磷酸化酶基因（LgUGPase），并通过对转基因拟南芥的分析，证实了该基因在促进营养生长和纤维素合成中的作用。UDP-葡萄糖焦磷酸化酶（UGPase）是碳水化合物代谢中的重要调节酶，催化葡萄糖1-磷酸的可逆产生以及尿苷三磷酸转化为尿苷二磷酸葡萄糖和焦磷酸。本研究从兴安落叶松中克隆了落叶松尿苷磷酸酶（LgUGPase）基因。该基因的开放阅读框长度为1,443 bp，编码480个氨基酸，预测分子量为53.7 kDa，与火炬松（Pinus taeda）和西云杉（Picea sitchensis）的UGPase蛋白序列具有显著的相似性。半定量逆转录-聚合酶链反应表明，LgUGPase基因主要表达在落叶松茎，除了它的根和叶。Southern杂交分析表明，LgUGPase由两个基因编码。gmelinii基因组。LgUGPase的过表达促进了转基因拟南芥的营养生长，增加了可溶性糖和纤维素的含量，并增厚了薄壁细胞壁。结果表明，L. gmelinii UGPase参与蔗糖/多糖代谢和细胞壁生物合成，提示LgUGPase可能是一个很好的改善植物纤维细胞发育的候选基因。
A UDP-glucose pyrophosphorylase gene ( LgUGPase ) was identified from Larix gmelinii, and its function in enhancing vegetative growth and cellulose biosynthesis was confirmed by analyzing transgenic Arabidopsis thaliana overexpressed LgUGPase . UDP-glucose pyrophosphorylase (UGPase), an important regulatory enzyme in carbohydrate metabolism, catalyzes the reversible production of glucose 1-phosphate and the conversion of uridine triphosphate to uridine diphosphate glucose and pyrophosphate. In this study, a larch UGPase (LgUGPase) gene was isolated from Larix gmelinii. The 1,443-bp open reading frame encodes a protein of 480 amino acids with a predicted molecular weight of 53.7 kDa and shows striking sequence similarity to UGPase proteins from Pinus taeda and Picea sitchensis. Semiquantitative reverse transcription-polymerase chain reaction showed that the LgUGPase gene was expressed primarily in the larch stem in addition to its root and leaf. Southern blot analysis indicated that LgUGPase is encoded by two genes in the L. gmelinii genome. Overexpression of LgUGPase enhanced vegetative growth in transgenic Arabidopsis and increased the contents of soluble sugars and cellulose, and thickened parenchyma cell walls. These results revealed that L. gmelinii UGPase participates in sucrose/polysaccharide metabolism and cell wall biosynthesis, suggesting that LgUGPase may be a good candidate gene for improvement of fiber cell development in plants.