A study of cytokeratin profiles in localized cutaneous amyloids
A study of cytokeratin profiles in localized cutaneous amyloids
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DOI：
10.1007/s00403-004-0474-3
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发表时间：
2004-04-01
影响因子：
3
通讯作者：
Tsai, SF
中科院分区：
文献类型：
--
作者：
Chang, YT;Liu, HN;Tsai, SF
局部皮肤淀粉样蛋白的主要成分可能来源于细胞角蛋白（CK）。然而，原发性皮肤淀粉样变性（PCA）和继发性皮肤淀粉样变性（SCA）的CK谱仍然不清楚。使用12种不同的多克隆或单克隆抗CK抗体（34 β E12、MNF 116、LP 34、AE 1/AE 3、抗CK 1、CK 5、CK 6、CK 7、CK 10、CK 14、CK 16和CK 17）对64例PCA、111例SCA和3例系统性淀粉样变性患者的皮肤组织石蜡包埋切片进行化学分析。此外，12例PCA患者的冷冻皮肤组织进行了分析，与石蜡包埋组织进行比较。在所有64个PCA石蜡切片中，淀粉样蛋白沉积物抗CK 5抗体和34 β E12免疫阳性。在所有12个PCA冷冻切片中，淀粉样蛋白沉积物抗CK 5抗体、34 β E12、MNF 116和LP 34免疫阳性，7个（58.3%）、3个（25%）和1个（8.3%）抗CK 1、CK 14和CK 10抗体免疫阳性。在所有SCA切片中，淀粉样蛋白沉积物对CK 5和34 β E12呈免疫阳性。此外，MNF 116免疫标记的淀粉样蛋白来自基底细胞癌和乳腺上皮瘤患者的所有切片，MNF 116和LP 34免疫标记的淀粉样蛋白来自汗孔角化病患者的切片。我们的研究结果表明，CK 5是目前在PCA和SCA的淀粉样蛋白沉积的主要CK，和“淀粉样蛋白-K”主要来自基底角质形成细胞。
The major component of localized cutaneous amyloids may be derived from cytokeratin (CK). However, the CK profiles of primary cutaneous amyloidosis (PCA) and secondary cutaneous amyloidosis (SCA) remain obscure. Paraffin-embedded sections of skin tissue from 64 patients with PCA, 111 with SCA and 3 with systemic amyloidosis were analyzed immunohistochemically using 12 different polyclonal or monoclonal anti-CK antibodies (34betaE12, MNF116, LP34, AE1/AE3, anti-CK1, CK5, CK6, CK7, CK10, CK14, CK16 and CK17). In addition, frozen skin tissues from 12 patients with PCA were analyzed for comparison with the paraffin-embedded tissue. In all 64 PCA paraffin sections, the amyloid deposits were immunopositive for anti-CK5 antibody and 34betaE12. In all 12 frozen sections of PCA, the amyloid deposits were immunopositive for anti-CK5 antibody, 34betaE12, MNF116 and LP34, and seven (58.3%), three (25%) and one (8.3%) were immunopositive for anti-CK1, CK14, and CK10 antibodies, respectively. In all SCA sections, the amyloid deposits were immunopositive for CK5 and 34betaE12. In addition, MNF116 immunolabeled amyloids of all sections from patients with basal cell carcinoma and trichoepithelioma, and MNF116 and LP34 immunolabeled amyloids of sections from patients with porokeratosis. Our results indicate that CK5 is the major CK present in the amyloid deposits of PCA and SCA, and "amyloid-K" is mainly derived from basal keratinocytes.