Chemically deglycosylated human chorionic gonadotropin subunits: characterization and biological properties.
Chemically deglycosylated human chorionic gonadotropin subunits: characterization and biological properties.
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化学去糖基化的人绒毛膜促性腺激素亚基：表征和生物学特性。
DOI：
10.1021/bi00282a007
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Ryan,RJ
中科院分区：
文献类型：
--
作者：
Keutmann,HT;McIlroy,PJ;Bergert,ER;Ryan,RJ
激素制剂的材料与方法。从Organon， Ltd （Oss, thenelands）购买部分纯化形式的hCG，通过Sephadex G-100凝胶过滤（Pharmacia）和deae -纤维素层析（Whatman）进行纯化。亚基的分离采用10 M尿素处理，然后采用deae -纤维素/3 M尿素色谱和Sephadex G-100凝胶过滤（Morgan et al., 1973）。纯化的hCG (CR-117和123),Dr. Robert Canfield （Columbia University, New York）赠送，用作生物学和免疫学检测的参比制剂。Deglycosylation。分别用亚基和全hCG进行氟化氢处理。5-10毫克肽在五氧化二磷上真空干燥24小时，转移到Toho聚丙烯蒸馏装置（Toho Industries, Tokyo, Japan），用10ml无水氟化氢（matheson, East Rutherford， NJ）在2.2 mL (20%) anisóle（用作清除剂）存在下处理（Mort & Lamport, 1977）。在0℃（冰浴保持）下反应1-3小时后，试剂蒸发。反应产物呈黄褐色颗粒状，用冷无水醚漂洗两次，然后悬浮在10ml 10%醋酸中。用额外的乙酸冲洗几次后，离心分离法去除少量不溶性颗粒
Materials and Methods Hormone Preparations. hCG purchased in partially purified form from Organon, Ltd.(Oss, TheNetherlands), was pro-cessed to purity by Sephadex G-100 gel filtration (Pharmacia) and DEAE-cellulose chromatography (Whatman). Subunits were separated by treatment with 10 M urea followed by DEAE-cellulose/3 M urea chromatography and Sephadex G-100 gel filtration (Morgan et al., 1973). Purified hCG (CR-117 and-123), a gift of Dr. Robert Canfield (Columbia University, New York), was used as reference preparation in the biological and immunological assays. Deglycosylation. Hydrogen fluoride treatment was carried out with the respective subunits and whole hCG. An aliquot of 5-10 mg of peptide was dried in vacuo over phosphorus pentoxide for 24 h, transferred to the Toho polypropylene distillation apparatus (Toho Industries, Tokyo, Japan), and treated with 10 mL of anhydrous hydrogen fluoride (Math-eson, East Rutherford, NJ) in thepresence of 2.2 mL (20%) of anisóle which was used as a scavenger (Mort & Lamport, 1977). After 1-3-h reaction at 0 C (maintained by ice bath) the reagents were evaporated. The granular, yellow-brown reaction product was rinsed twice with cold anhydrous ether and then suspended in 10 mL of 10% acetic acid. After several rinses with additionalacetic acid, the preparation was cen-trifuged to remove a small amount of insoluble particulate
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绵羊垂体促黄体素亚基的去糖基化：对亚基相互作用和激素活性的影响。
影响因子：
3.9
作者：
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通讯作者：
M. Sairam
[19] 糖蛋白中碳水化合物的气相色谱分析。
影响因子：
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作者：
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还原蛋白和 S-羧甲基化蛋白的制备和酶水解。
影响因子：
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作者：
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卵巢受体与人黄体生成激素和人绒毛膜促性腺激素的相互作用。
DOI：
--
发表时间：
1973
期刊：
影响因子：
--
作者：
Lee Cy;Ryan Rj
通讯作者：
Ryan Rj
人绒毛膜促性腺激素。
DOI：
10.1016/s0021-9258(19)63361-2
发表时间：
1977
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Keutmann;R. Williams
通讯作者：
R. Williams