Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther).
Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther).
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DOI：
10.1590/s1517-838220120001000046
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发表时间：
2012-01
期刊：
影响因子：
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通讯作者：
Tang JF
中科院分区：
文献类型：
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作者：
Cai SH;Wu ZH;Jian JC;Lu YS;Tang JF
从20份长吻鲶样品中分离到273株细菌。所有菌株的生化特性均符合维氏气单胞菌的种描述。Veronii基于Vitek GNI+卡。此外，对代表菌株PY 50的16 S rDNA、gyrB和rpoD序列进行了测定，结果表明PY 50与A.韦罗尼公司veronii的序列。采用Kirby-Bauer纸片扩散法对代表菌株PY 50进行了药敏试验，结果表明，该菌株对21种抗菌药物中的13种和4种均为敏感和中度敏感。菌株PY 50的胞外产物中含有明胶酶、卵磷脂酶、弹性蛋白酶以及大部分脂肪酶和脂多糖。测定了PY 50菌株及其胞外产物对中华长吻鲶的毒力，腹腔注射LD 50分别为3.47×104 CFU/尾和11.22 μg/尾。这是A.韦罗尼公司veronii是长吻鲶溃疡综合征的病原。
273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv. veronii in Genbank. Antibiotic-resistance of the representative strain PY50 was assessed by the Kirby-Bauer disk diffusion method, and the results showed it was susceptible and moderately susceptible to 13 and 4 of the 21 antimicrobial agents tested. Extracellular products of strain PY50 contained gelatinase, lecithinase, elastase, most of lipase and lipopolysaccharide. Virulence of strain PY50 and extracellular products to Chinese longsnout catfish were also tested, and LD50 were about 3.47×104 CFU per fish and 11.22 μg per fish in intraperitoneal injection respectively. This is the first report that A. veronii bv. veronii was the pathogenic agent of ulcerative syndrome in Chinese longsnout catfish.