Characterization of middle-molecule introduction into cells using mm-scale discharge in saline
Characterization of middle-molecule introduction into cells using mm-scale discharge in saline
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DOI：
10.35848/1347-4065/ab7d7e
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发表时间：
2020-04-01
影响因子：
1.5
通讯作者：
Kaneko, Toshiro
中科院分区：
文献类型：
--
作者：
Honda, Ryosuke;Sasaki, Shota;Kaneko, Toshiro
虽然气相大气压等离子体（APP）是一种用于高效和微创基因/药物（分子）导入的有前途的技术，但是用于体内治疗的外部气体供应的减少和生物流体中的有效治疗区域的扩展仍然具有挑战性。我们开发了一种在盐水中产生毫米级等离子体的装置，无需工作气体供应，并证明了使用该装置将YOYO-1分子（分子量：1271）局部引入培养细胞。与传统的APP不同，等离子体诱导的机械刺激是分子引入的关键因素之一。
While gas-phase atmospheric pressure plasma (APP) is a promising technology for highly-efficient and minimally-invasive gene/drug (molecule) introduction, the reduction of external gas supply and the expansion of the effective treatment area in biological fluids for in vivo treatment are still challenging. We developed a device for mm-scale plasma generation in saline without working gas supply and demonstrated the local introduction of YOYO-1 molecule (molecular weight : 1271) into cultured cells using the device. Unlike the conventional APP accounting for chemical stimuli with reactive species, plasma-induced mechanical stimulus was indicated as one of key factor(s) in the molecule introduction.