SFC-APLI-(TOF)MS: Hyphenation of Supercritical Fluid Chromatography to Atmospheric Pressure Laser Ionization Mass Spectrometry.
SFC-APLI-(TOF)MS: Hyphenation of Supercritical Fluid Chromatography to Atmospheric Pressure Laser Ionization Mass Spectrometry.
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DOI：
10.1021/acs.analchem.5b04402
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发表时间：
2016-01
影响因子：
7.4
通讯作者：
Dennis Klink;O. Schmitz
中科院分区：
文献类型：
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作者：
Dennis Klink;O. Schmitz
大气压激光电离质谱（APLI-MS）是分析多环芳烃（PAH）分子的一种强有力的方法，它通过共振增强的多光子电离具有选择性和高灵敏度。APLI于2005年提出，并成功地与高效液相色谱（HPLC）和气相色谱（GC）等色谱分离技术相结合。为了将色谱耦合扩展到APLI，建立了APLI与超临界流体色谱（SFC）的连接装置。在这里，我们以一种简单的设计方式展示了SFC和APLI的第一个连字符，涉及不同的优化步骤，以确保敏感的分析。新的设置允许定性和定量测定原生和更极性多环芳烃分子。由于源内环境特性的改变，人尿中1-羟基芘（1-HP）的定量可以在没有事先衍生化的情况下进行。sfc - apl - tof （MS）对1-HP的检出限为0.5 μ L(-1)，低于接触者1-HP的检出限。
Atmospheric-pressure laser ionization mass spectrometry (APLI-MS) is a powerful method for the analysis of polycyclic aromatic hydrocarbon (PAH) molecules, which are ionized in a selective and highly sensitive way via resonance-enhanced multiphoton ionization. APLI was presented in 2005 and has been hyphenated successfully to chromatographic separation techniques like high performance liquid chromatography (HPLC) and gas chromatography (GC). In order to expand the portfolio of chromatographic couplings to APLI, a new hyphenation setup of APLI and supercritical-fluid chromatography (SFC) was constructed and aim of this work. Here, we demonstrate the first hyphenation of SFC and APLI in a simple designed way with respect to different optimization steps to ensure a sensitive analysis. The new setup permits qualitative and quantitative determination of native and also more polar PAH molecules. As a result of the altered ambient characteristics within the source enclosure, the quantification of 1-hydroxypyrene (1-HP) in human urine is possible without prior derivatization. The limit of detection for 1-HP by SFC-APLI-TOF(MS) was found to be 0.5 μg L(-1), which is lower than the 1-HP concentrations found in exposed persons.