A novel lineage of candidate pheromone receptors for sex communication in moths
A novel lineage of candidate pheromone receptors for sex communication in moths
复制标题
DOI：
10.7554/elife.49826
复制
发表时间：
2019-12-10
期刊：
影响因子：
7.7
通讯作者：
Montagne, Nicolas
中科院分区：
文献类型：
--
作者：
Bastin-Heline, Lucie;de Fouchier, Arthur;Montagne, Nicolas
性信息素受体（PR）是昆虫交配伴侣之间化学通讯的关键参与者。在高度多样化的昆虫目的鳞翅目，男性的PR调谐到女性发出的I型信息素（其中占绝大多数的信息素确定）形成一个专门的亚家族的气味受体（OR）。在这里，使用异源表达和体内基因组编辑方法的组合，我们带来了功能性证据，证明至少有一个蛾PR不属于这个亚科，而是属于一个远亲OR谱系。这种PR，在棉夜蛾（Spodoptera littoralis）中鉴定，在雄性触角中高度表达，并且专门调谐到雌性发出的主要性信息素成分。结合对蛾类信息素的系统发育分析，我们的功能数据表明，在鳞翅目昆虫中存在两个独立的信息素受体，为研究蛾类信息素通讯的进化提供了一条新的途径。
Sex pheromone receptors (PRs) are key players in chemical communication between mating partners in insects. In the highly diversified insect order Lepidoptera, male PRs tuned to female-emitted type I pheromones (which make up the vast majority of pheromones identified) form a dedicated subfamily of odorant receptors (ORs). Here, using a combination of heterologous expression and in vivo genome editing methods, we bring functional evidence that at least one moth PR does not belong to this subfamily but to a distantly related OR lineage. This PR, identified in the cotton leafworm Spodoptera littoralis, is highly expressed in male antennae and is specifically tuned to the major sex pheromone component emitted by females. Together with a comprehensive phylogenetic analysis of moth ORs, our functional data suggest two independent apparitions of PRs tuned to type I pheromones in Lepidoptera, opening up a new path for studying the evolution of moth pheromone communication.