Association of Interleukin-10 gene promoter polymorphisms in Saudi patients with vitiligo
Association of Interleukin-10 gene promoter polymorphisms in Saudi patients with vitiligo
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DOI：
10.1155/2008/210609
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Tariq, Mohammad
中科院分区：
文献类型：
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作者：
Abanmi, Abdullah;Al Harthi, Fahad;Tariq, Mohammad
人类白细胞介素10基因的启动子区具有高度多态性，与多种自身免疫性疾病有关。最近的研究将白癜风与自身免疫系统缺陷联系起来。本研究旨在探讨沙特人群中IL-10基因多态性与白癜风之间可能的关联。这项病例对照研究由 184 名沙特受试者组成，其中包括 83 名白癜风患者（40 名男性，43 名女性，平均年龄 27.85 +/- 12.43 岁）和 101 名匹配对照。使用 QIA amp(R) DNA 迷你试剂盒（Qiagen CA，美国）从利雅得武装部队医院皮肤科门诊就诊的健康对照者和白癜风患者的血液样本中提取基因组 DNA。使用Arms引物通过聚合酶链反应（PCR）扩增IL-10基因，检测592、-819和-1082位点的多态性。与健康受试者相比，白癜风患者中1082位点的GG基因型以及592和819位点的CC基因型频率显着高于健康受试者，表明沙特人的GG和CC基因型可能易患白癜风。另一方面，IL-10 的基因型-1082 GA、-819 CT 和-592 CA 在健康对照中更为普遍，表明 GA、CT 和 CA 基因型对白癜风具有保护作用。这项研究表明 IL-10 基因可能在沙特人白癜风的病因学中发挥重要作用。
The promoter region of human Interleukin-10 gene is highly polymorphic and has been associated with numerous autoimmune diseases. Recent studies have linked vitiligo with defective autoimmune system. This study is aimed to explore a possible association between IL-10 gene polymorphism and vitiligo in Saudi population. This case control study consisted of 184 Saudi subjects including 83 vitiligo patients (40 males, 43 females mean age 27.85 +/- 12.43 years) and 101 matched controls. Genomic DNA was extracted from the blood samples of healthy controls and Vitiligo patients visiting out patient clinic of Department of Dermatology, Riyadh Armed Forces Hospital, using QIA amp(R) DNA mini kit (Qiagen CA, USA). Interleukin-10 gene was amplified by polymerase chain reaction (PCR) using Arms primers to detect any polymorphism involved at positions-592,-819 and-1082.The frequencies of GG genotype at-1082, and CC genotype at positions-592 and 819 were significantly higher in vitiligo patients compared to healthy subjects suggesting that GG and CC genotypes might be susceptible to vitiligo in Saudis. On the other hand genotypes-1082 GA,-819 CT, and-592 CA of IL-10 were more prevalent in healthy controls suggesting protective effects of GA, CT and CA genotypes against vitiligo. This study indicates that the IL-10 gene may play a significant role in the etiology of vitiligo among Saudis.