Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri
Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri
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DOI：
10.1016/j.jprot.2011.05.028
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发表时间：
2011-09-06
影响因子：
3.3
通讯作者：
Millar, Andrew J.
中科院分区：
文献类型：
--
作者：
Le Bihan, Thierry;Martin, Sarah F.;Millar, Andrew J.
牛链球菌是一种单细胞绿色球菌，是最小和最简单的自由生活的真核生物之一。手术2006年确定了金牛座的基因组序列。从那时起产生的分子，生理和分类数据突出了它作为藻类和植物简单模式物种的潜力。然而，它的蛋白质组仍然在很大程度上未被探索。本文综述了O. tauri，使用基于质谱的方法：磷酸肽富集，细胞分级分离，无标记定量和N-15代谢标记。手术在以下条件下分析Tauri蛋白质组：在昼夜节律周期的不同时间、光照24小时后、黑暗24小时后和各种氮源供应水平下取样。细胞周期相关蛋白如发动蛋白和驱动蛋白在光暗转换过程中显著上调。这反映在它们在ZT 13处的较高强度，并且该过渡阶段与有丝分裂的结束一致。参与几种代谢机制的蛋白质在低氮条件下被发现上调，包括碳储存途径，糖酵解，磷酸盐转运和无机多磷酸盐的合成。牡蛎响应低氮条件下，减少其氮同化机制，这表明一个非典型的适应机制，以应对营养有限的环境。(C)2011 Elsevier B. V.保留所有权利。
Ostreococcus tauri is a unicellular green alga and amongst the smallest and simplest free-living eukaryotes. The O. tauri genome sequence was determined in 2006. Molecular, physiological and taxonomic data that has been generated since then highlight its potential as a simple model species for algae and plants. However, its proteome remains largely unexplored. This paper describes the global proteomic study of O. tauri, using mass spectrometry-based approaches: phosphopeptide enrichment, cellular fractionation, label-free quantification and N-15 metabolic labeling. The O. tauri proteome was analyzed under the following conditions: sampling at different times during the circadian cycle, after 24 h of illumination, after 24 h of darkness and under various nitrogen source supply levels. Cell cycle related proteins such as dynamin and kinesin were significantly up-regulated during the daylight-to-darkness transition. This is reflected by their higher intensity at ZT13 and this transition phase coincides with the end of mitosis. Proteins involved in several metabolic mechanisms were found to be up-regulated under low nitrogen conditions, including carbon storage pathways, glycolysis, phosphate transport, and the synthesis of inorganic polyphosphates. Ostreococcus tauri responds to low nitrogen conditions by reducing its nitrogen assimilation machinery which suggests an atypical adaptation mechanism for coping with a nutrient-limited environment. (C) 2011 Elsevier B.V. All rights reserved.