High expression of Wnt7b in human superficial bladder cancer vs invasive bladder cancer.
High expression of Wnt7b in human superficial bladder cancer vs invasive bladder cancer.
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DOI：
10.1038/bjc.1998.49
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发表时间：
1998
影响因子：
8.8
通讯作者：
Harris, A L
中科院分区：
文献类型：
--
作者：
Bui, T D;O'Brien, T;Crew, J;Cranston, D;Harris, A L
Wnt基因的异常表达与乳腺癌的发生发展有关，但它在其他肿瘤中的作用尚不清楚。WNTS调节钙粘附素的功能，以前显示在浸润性膀胱癌中更常见的是去调节。本研究调查了钙粘附素上游因子在浅表性膀胱癌中是否异常表达。用核糖核酸酶保护法分析了4株人膀胱癌细胞系、8例正常膀胱组织和48例膀胱癌组织中Wnt7b和Wnt5a基因的表达。所有细胞株的WNT7b基因表达水平基本相同。Wnt5A和Wnt7b在正常膀胱组织和膀胱肿瘤组织中均有表达。Wnt7b在浅表性肿瘤(n=29)中的表达中值是正常组织(n=8，P=0.002)的4倍，是浸润性肿瘤(n=17，P=0.003)的5倍。正常组织与浸润性肿瘤之间的表达差异无统计学意义(P=0.3)。Wnt5a在正常组织与浅表肿瘤(P=0.4)、正常组织与浸润性肿瘤(P=0.3)、浅表性肿瘤与浸润性肿瘤(P=0.2)之间的表达差异无统计学意义。Wnt7b的差异表达提示在浅表性膀胱肿瘤发生的早期事件中发挥了作用，涉及细胞黏附，并为浅表性和浸润性癌症不同的进化途径提供了进一步的证据。
Aberrant Wnt gene expression is involved in the development of breast cancer, but its role in other tumours is unknown. Wnts regulate cadherin function, previously shown to be more commonly deregulated in invasive bladder cancer. This study investigated whether factors upstream of cadherins were aberrantly expressed in superficial bladder cancer. The expression of one transforming (Wnt7b) and one non-transforming (Wnt5a) Wnt gene in four human bladder carcinoma cell lines, and in normal human bladder tissues (n = 8) and bladder cancers (n = 48) were analysed by ribonuclease protection analysis. All cell lines expressed an approximately equal level of Wnt7b mRNA. Wnt5a and Wnt7b mRNAs were both expressed in normal bladder tissues and bladder tumours. The median expression of Wnt7b was fourfold higher in superficial tumours (n = 29) than in normal tissues (n = 8, P = 0.002) and five fold higher than in invasive tumours (n = 17, P = 0.003). There was no significant difference between normal tissues and invasive tumours (P = 0.3). The expression of Wnt5a did not vary significantly between normal tissues and superficial tumours (P = 0.4), normal tissues and invasive tumours (P = 0.3) or superficial tumours and invasive tumours (P = 0.2). The differential expression of Wnt7b suggests a role in the early events of superficial bladder tumorigenesis involving cell adhesion and provides further evidence of different pathways of evolution of superficial and invasive cancer.