Plasma levels of intact and degraded ghrelin and their responses to glucose infusion in anorexia nervosa
Plasma levels of intact and degraded ghrelin and their responses to glucose infusion in anorexia nervosa
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DOI：
10.1210/jc.2004-0353
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发表时间：
2004-11-01
影响因子：
5.8
通讯作者：
Takano, K
中科院分区：
文献类型：
--
作者：
Hotta, M;Ohwada, R;Takano, K
辛酰化的生长素释放肽（1-28）（完整的生长素释放肽）快速且容易降解为脱辛酰形式或更小的片段（降解的生长素释放肽）。采用几种测定方法检测了30名神经性厌食症（AN）患者（体重指数，8.81-22.4 kg/m2）和16名年龄匹配的健康女性的完整和降解ghrelin的血浆水平。血浆ghrelin水平测定免疫复合物转移酶免疫分析，专门检测完整的ghrelin，AN低于对照组。使用活性生长素释放肽ELISA试剂盒测量AN中的血浆生长素释放肽水平，该试剂盒被宣传为特异性检测完整的生长素释放肽，与对照组没有显著差异。血浆去辛酰ghrelin使用去酰基ghrelin ELISA试剂盒，N-末端ghrelin使用ghrelin活性RIA试剂盒，和C-末端ghrelin使用ghrelin总RIA试剂盒的水平显着高于AN比对照组，并显示出显着的负相关性与体重指数。在静脉葡萄糖输注过程中使用免疫复合物转移酶免疫测定或活性ghrelin ELISA测定的ghrelin血浆水平在AN和对照组中均受到抑制，而AN中降解的ghrelin血浆水平没有显著降低。因此，AN中完整的生长素释放肽的血浆水平并不高于对照组，而AN中生长素释放肽的降解形式升高。葡萄糖输注后，AN患者血浆中完整的生长素释放肽（而非降解的生长素释放肽）迅速受到抑制。AN患者血浆中完整和降解形式的ghrelin的概况与健康女性不同。
Octanoylated ghrelin (1-28) ( intact ghrelin) is rapidly and easily degraded to desoctanoyl forms or smaller fragments (degraded ghrelin). Plasma levels of intact and degraded ghrelin were examined in 30 patients with anorexia nervosa (AN) ( body mass index, 8.81-22.4 kg/m(2)) and 16 age-matched healthy women using several assay methods. Plasma levels of ghrelin measured using immunocomplex transfer-enzyme immunoassay, which specifically detects intact ghrelin, were lower in AN than controls. Plasma ghrelin levels in AN measured using the active ghrelin ELISA kit, which is advertised as specifically detecting intact ghrelin, did not differ significantly from controls. Plasma levels of desoctanoyl ghrelin using the desacyl-ghrelin ELISA kit, N-terminus ghrelin using the ghrelin active RIA kit, and C-terminus ghrelin using the ghrelin total RIA kit were significantly higher in AN than controls, and displayed significant negative correlations with body mass index. Plasma levels of ghrelin determined using immunocomplex transfer-enzyme immunoassay or active ghrelin ELISA during iv glucose infusion were suppressed in both AN and controls, whereas plasma levels of degraded ghrelin levels were not significantly decreased in AN. Plasma levels of intact ghrelin are therefore not higher in AN than controls, whereas degraded forms of ghrelin are elevated in AN. Rapid suppression of plasma intact ghrelin, but not degraded ghrelin, occurs in AN in response to glucose infusion. The profiles of intact and degraded forms of ghrelin in plasma of AN patients differ from those of healthy women.