Qualitative Identification of Dibenzoylmethane in Licorice Root (Glycyrrhiza glabra) using Gas Chromatography-Triple Quadrupole Mass Spectrometry
Qualitative Identification of Dibenzoylmethane in Licorice Root (Glycyrrhiza glabra) using Gas Chromatography-Triple Quadrupole Mass Spectrometry
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DOI：
10.1177/1934578x1400900127
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发表时间：
2014-01
影响因子：
1.8
通讯作者：
M. Mancia;M. Reid;Evan S. Dubose;J. Campbell;K. Jackson
中科院分区：
文献类型：
--
作者：
M. Mancia;M. Reid;Evan S. Dubose;J. Campbell;K. Jackson
甘草是一种中草药，在治疗和癌症预防方面具有药用价值。二苯甲酰甲烷(DBM；1，3-二苯基-1，3-丙二酮)是一种小的β-二酮，据报道是甘草的一种次要成分，并已知是人类前列腺癌细胞周期的去调节因子。表征甘草根部形态的植物化学特征，包括商业上可用的DBM，将促进我们寻找前列腺癌治疗的新试剂。采用气相色谱-三重四极杆-质谱法测定甘草提取物中的DBM。DBM和所有甘草形式都显示出保留时间为14.5分钟的成分。选择性反应监测/SRM在确定的保留时间检测到的主要碎片离子为m/z 77、105、147、223和224。这些数据证实了天然来源的DBM的存在，GC-MS/SRM方法有助于鉴定复杂生物基质中的这一次要成分。
Licorice root (Glycyrrhiza glabra), an herbal Chinese medicine, has shown medicinal uses in therapeutics and cancer prevention. Dibenzoylmethane (DBM; 1, 3-diphenyl-1, 3-propadinedione), a small β-diketone, has been reported to be a minor constituent of licorice and a known deregulator of the human prostate cancer cell cycle. Characterization of the phytochemical profiles of licorice root forms including commercially available DBM will advance our search in identifying novel reagents for prostate cancer therapeutics. Gas chromatography- triple quadrupole-mass spectrometric analysis was used for detecting DBM in licorice root extracts. DBM and all licorice forms exhibited a component with a retention time of 14.5 minutes. The major fragment ions detected were at m/z 77, 105, 147, 223 and 224 at the identified retention time by selected reaction monitoring/SRM. These data confirm the presence of DBM from its natural source (G. glabra), and the GC-MS/SRM method helps in the identification of this minor component in a complex biological matrix.