HUMAN ALPHA-FETOPROTEIN AS A MODULATOR OF HUMAN LYMPHOCYTE-TRANSFORMATION - CORRELATION OF BIOLOGICAL POTENCY WITH ELECTROPHORETIC VARIANTS
HUMAN ALPHA-FETOPROTEIN AS A MODULATOR OF HUMAN LYMPHOCYTE-TRANSFORMATION - CORRELATION OF BIOLOGICAL POTENCY WITH ELECTROPHORETIC VARIANTS
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DOI：
10.1073/pnas.73.12.4645
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
YACHNIN, S
中科院分区：
文献类型：
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作者：
LESTER, EP;MILLER, JB;YACHNIN, S
人类.alpha。免疫吸附柱分离的-胎蛋白（HAFP）可抑制人淋巴细胞对植物原[豆豆蛋白A、植物血凝素、美洲商陆有丝分裂原]、抗人胸腺细胞抗血清和混合淋巴细胞培养物的有丝分裂反应。从5例肝癌患者的血清、腹水和胎儿肝脏中分离的HAFP的生物学效力差异超过3个数量级。扩展琼脂糖凝胶电泳和交叉免疫电泳证实了HAFP的3个分子种。对3种植物的定量分析表明，带负电荷最多的物种的相对数量与生物效力之间存在相关性。用神经氨酸酶处理HAFP以完全去除唾液酸残基并没有改变其生物效力，而是将3种转化为2种，其电泳迁移速度较慢。唾液酸含量的差异只是HAFP显示的微异质性的部分原因，另一个带电部分的变异性也存在。HAFP不同分子种类相对比例的变化可能在其免疫抑制特性的调节中起重要作用。
Human .alpha.-fetoprotein (HAFP) isolated by immunoadsorbent column suppressed the mitogenic response of human lymphocytes to phytomitogens [concanavalin A, phytohemagglutinin, pokeweed mitogen], antihuman thymocyte antiserum and the mixed lymphocyte culture. HAFP isolated from the sera and ascitic fluid of 5 hepatoma patients and from fetal liver varied in biological potency over 3 orders of magnitude. Extended agarose gel electrophoresis and crossed immunoelectrophoresis demonstrated 3 molecular species of HAFP. Quantitation of the 3 species revealed a correlation between the relative amount of the most negatively charged species and biological potency. Treatment of HAFP with neuraminidase to completely remove sialic acid residues did not alter the biological potency, but converted the 3 species to 2 species having slower electrophoretic mobilities. Differences in sialic acid content are only partly responsible for the microheterogeneity demonstrated by HAFP, and that variability in another charged moiety is also present. Variation in the relative proportions of the different molecular species of HAFP may be important in the regulation of its immunosuppressive properties.