Honokiol Improves Insulin Resistance, Hepatic Steatosis, and Inflammation in Type 2 Diabetic db/db Mice
Honokiol Improves Insulin Resistance, Hepatic Steatosis, and Inflammation in Type 2 Diabetic db/db Mice
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DOI：
10.3390/ijms20092303
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发表时间：
2019-05-01
影响因子：
5.6
通讯作者：
Jung, Un Ju
中科院分区：
文献类型：
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作者：
Kim, Young-Je;Jung, Un Ju
本研究的重点是和厚朴酚（HON）对2型糖尿病db/db小鼠的葡萄糖稳态，胰岛素抵抗，血脂异常，肝脂肪变性和炎症的影响。雄性C57 BL/KsJ-db/db小鼠喂食含有或不含HON（0.02%，w/w）或吡格列酮（PIO，抗糖尿病剂，0.01%，w/w）的正常饮食5周。测定血液生物标志物、组织形态、酶和遗传参数。PIO显著降低食物摄入量、空腹血糖和糖化血红蛋白（HbA 1c）水平，但显著增加体重、脂肪组织重量和血浆瘦素水平。HON对摄食量、体重、血浆瘦素和血糖水平无显著影响。然而，HON导致脂肪组织重量、血浆胰岛素、血液HbA 1c和HOMA-IR水平显著降低，并改善葡萄糖耐量。HON的抗糖尿病和抗肥胖作用分别部分与抑制肝脏中的生脂酶及其mRNA表达和抑制脂肪组织中的生脂酶有关。与PIO不同，HON不影响血脂异常，但通过抑制肝脂肪生成酶活性来改善肝脂肪变性。此外，HON表现出与PIO相似的抗炎作用。这些结果表明，HON可以通过改善胰岛素抵抗、糖和脂质代谢以及炎症来预防2型糖尿病。
This study focuses on the effect of honokiol (HON) on glucose homeostasis, insulin resistance, dyslipidemia, hepatic steatosis, and inflammation in type 2 diabetic db/db mice. Male C57BL/KsJ-db/db mice were fed a normal diet with or without HON (0.02%, w/w) or pioglitazone (PIO, anti-diabetic agent, 0.01%, w/w) for 5 weeks. Blood biomarker, tissue morphology and enzymatic and genetic parameters were determined. PIO significantly decreased food intake, fasting blood glucose, and glycosylated hemoglobin (HbA1c) levels, but markedly increased body weight, adipose tissue weight, and plasma leptin levels. HON did not significantly affect food intake, body weight, or levels of plasma leptin and blood glucose. However, HON led to significant decreases in adipose tissue weight, plasma insulin, blood HbA1c and HOMA-IR levels and improved glucose tolerance. The anti-diabetic and anti-adiposity effects of HON were partially related to the inhibition of gluconeogenic enzymes and their mRNA expression in the liver; and the inhibition of lipogenic enzymes in adipose tissue, respectively. Unlike PIO, HON did not affect dyslipidemia, but ameliorated hepatic steatosis by inhibiting hepatic lipogenic enzymes activity. Moreover, HON exhibited anti-inflammatory effects similar to PIO. These results suggest that HON can protect against type 2 diabetes by improving insulin resistance, glucose and lipid metabolism, and inflammation.