Disulfide linkages between antigen-binding receptors on chicken B-lymphocytes.
Disulfide linkages between antigen-binding receptors on chicken B-lymphocytes.
复制标题
鸡 B 淋巴细胞上抗原结合受体之间的二硫键。
DOI：
10.1016/0161-5890(86)90166-5
复制
发表时间：
1986
影响因子：
3.6
通讯作者：
Choi,YS
中科院分区：
文献类型：
--
作者：
Rosenspire,AJ;Lee,MS;Pollak,SV;Choi,YS
Membrane immunoglobulin receptors on chicken B-cells have been shown to display a heterogeneity with respect to interchain disulfide linkages. One fraction of the surface Ig (sIg) appears to display the traditional H2-L2linkage. We also present evidence that this Ig is covalently bound via a disulfide linkage to actin. In this instance, the isolated Ig heavy chain, after reduction, has a mol. wt of 80 K. Perhaps more significantly, we show that another fraction of the sIg exists in a highly aggregated form that is stabilized by disulfide linkages. In contrast to the sIg found in the H2-L2configuration, there is no evidence of actin within the aggregates and the sIg heavy chains isolated from these aggregates display a slightly faster mobility on SDS-PAGE under reducing conditions, running at about 77 K. Furthermore, it appears that the Ig within the large aggregates may have a higher avidity with respect to antigen binding, and so this Ig structure may be the more relevant to antigen-induced receptor-mediated signaling in the B-cell.
