RELATIVE IMPORTANCE OF KIDNEY AND LIVER IN SYNTHESIS OF ARGININE BY RAT
RELATIVE IMPORTANCE OF KIDNEY AND LIVER IN SYNTHESIS OF ARGININE BY RAT
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DOI：
10.1152/ajplegacy.1973.224.1.127
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发表时间：
1973-01-01
影响因子：
--
通讯作者：
FREEDLAND, RA
中科院分区：
文献类型：
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作者：
FEATHERSTON, WR;ROGERS, QR;FREEDLAND, RA
Featherston，WR，Q. R.罗杰斯，和RA弗里兰。肾脏和肝脏在大鼠精氨酸合成中的相对重要性。Am. J.Physiol.224（1）：Y127 -129。1973.-在完整对照组或肾脏或肝脏或两者均完全结扎的大鼠中，通过跟踪静脉注射的L-瓜氨酸-脲基-14 C转化为L-精氨酸胍基-14 C，研究了肾脏和肝脏对大鼠精氨酸总合成的相对贡献。掺入标记精氨酸三氯乙酸沉淀蛋白从肝，肾，骨骼肌，脑，肺进行了测定。当肾脏结扎时，观察到骨骼肌、肺和肝脏蛋白比活性的降低幅度大于当肝脏结扎时骨骼肌、肺和肾脏蛋白的降低幅度。骨骼肌蛋白的比活性是约3.3倍时，肝脏被结扎时，肾脏被结扎。肾脏、肝脏或两者的连接对降低脑蛋白比活性的影响小于连接对骨骼肌蛋白比活性的影响，表明大脑可能从瓜氨酸合成其自身精氨酸的一部分。结果提供的证据表明，肾脏合成了相当大的一部分内源性精氨酸用于身体蛋白质合成比肝脏。骨骼肌;大脑;肺;瓜氨酸大鼠肾脏从瓜氨酸合成精氨酸的能力已得到充分证实（1，3，9）。Rogers等人(10)最近提出的证据表明，在肝脏中，瓜氨酸转化为精氨酸是有限的，合成的精氨酸大部分被分解为尿素和鸟氨酸。他们的工作表明，肾脏可能提供了组织合成所需的精氨酸的重要部分，这是肝脏产生的瓜氨酸在肾脏中合成精氨酸的结果。本研究旨在测量肾脏和肝脏对精氨酸全身合成的相对贡献。结果表明精氨酸合成的大脑内使用这个器官也提出。
FEATHERSTON, WR, Q. R. ROGERS, AND RA FREEDLAND. Relative importance of kidney and liver in synthesis of arginine by the rat. Am. J. Physiol. 224 (l): Yl27-129. 1973.-The relative contributions of the kidneys and liver to the total synthesis of arginine in the rat were studied by following the conversion of intravenously injected L-citrulline-ureido-14C to L-arginine guanido-14C in intact controls or in rats with either the kidneys, or liver, or both completely ligated. The incorporation of the labeled arginine into the trichloroacetic acid-precipitable protein from liver, kidney, skeletal muscle, brain, and lung was determined. A greater decrease in the specific activity of skeletal muscle, lung, and liver protein was observed when the kidneys were ligated than occurred in the skeletal muscle, lung, and kidney protein when the liver was ligated. The specific activity of skeletal muscle protein was approximately 3.3 times higher when the liver was ligated than when the kidneys were ligated. Ligation of either the kidney, liver, or both had less of an effect on lowering the specific activity of brain protein than the ligation had on the specific activity of skeletal muscle protein indicating the brain may be synthesizing a portion of its own arginine from citrulline. The results provide evidence that the kidneys synthesize a considerably larger portion of the endogenous arginine used for body protein synthesis than does the liver. skeletal muscle; brain; lung; citrullineTHE ABILITY OF THE KIDNEY of the rat to synthesize arginine from citrulline has been well established (1, 3, 9). Rogers et al.(10) have recently presented evidence that in the liver the conversion of citrulline to arginine is limited and that much of the arginine synthesized is broken down to urea and ornithine. Their work suggested that the kidney may be supplying a significant portion of the arginine needed for tissue synthesis as a result of arginine synthesis in the kidney from citrulline produced in the liver. The present study was designed to measure the relative contributions of the kidneys and liver to the total-body synthesis of arginine. Results indicating arginine synthesis by the brain for use within this organ are also presented.