CHARACTERIZATION OF RNA HAIRPIN LOOP STABILITY
CHARACTERIZATION OF RNA HAIRPIN LOOP STABILITY
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DOI：
10.1093/nar/16.24.11725
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发表时间：
1988-12-23
影响因子：
14.9
通讯作者：
UHLENBECK, OC
中科院分区：
文献类型：
--
作者：
GROEBE, DR;UHLENBECK, OC
合成了15个具有相同茎序列且带有由A、C和U残基组成的均聚物环（其长度从3到9个核苷酸不等）的RNA发夹结构，并测定了它们的热稳定性。熔解温度（Tm）随环的大小而变化，但几乎与环的组成无关。发现4个或5个核苷酸的环是最稳定的环大小。这与在16S样RNA中四元环是最常见的环大小这一观察结果是一致的。通过减去螺旋茎已知的贡献来计算每个环对发夹稳定性的贡献。这些数据对于预测其他发夹的稳定性应该是有用的。
Fifteen RNA hairpins that share the same stem sequence and have homopolymer loops of A, C and U residues which vary in length from three to nine nucleotides were synthesized and their thermal stabilities determined. Tm varys as a function of loop size but is almost independent of loop composition. Loops of four or five nucleotides are found to be the most stable loop size. This is consistent with the observation that four-membered loops are the most prevalent loop size in 16S-like RNAs. The contribution of each loop to hairpin stability was calculated by subtracting the known contribution of the helical stem. These data should be useful for predicting the stability of other hairpins.