THE CHOLINERGIC SYSTEM IN DEVELOPING CEREBELLUM - COMPARATIVE-STUDY OF NORMAL, HYPOTHYROID AND UNDERFED RATS
THE CHOLINERGIC SYSTEM IN DEVELOPING CEREBELLUM - COMPARATIVE-STUDY OF NORMAL, HYPOTHYROID AND UNDERFED RATS
复制标题
DOI：
10.1159/000111898
复制
发表时间：
1989-01-01
影响因子：
2.9
通讯作者：
GOMBOS, G
中科院分区：
文献类型：
--
作者：
CLOS, J;GHANDOUR, S;GOMBOS, G
为了克服以往发现的不足，我们在很短的年龄间隔内研究了胆碱乙酰转移酶(ChAT)和乙酰胆碱酯酶(AChE)的活性，以便更准确地确定正常大鼠、甲状腺功能低下大鼠和喂养不足大鼠的发育曲线的形状和时间。此外，我们还用组织化学方法研究了三种神经学模型小脑发育过程中AChE的表达。与文献中的结构结果相比较，这些结果提供了与小脑神经传递有关的正常和异常发育的胆碱能系统的以下信息：(1)与ChAT不同，AChE活性可被认为是发育中的胆碱能原脑的良好标志，(2)ChAT和AChE在功能性非胆碱能浦肯野细胞中短暂地同时表达。与中枢神经系统的大多数区域相比，小脑发育早期ChAT与AChE活性的高比率表明乙酰胆碱(Ach)的合成增强。ChAT活性水平与Purkinje细胞大小相关，支持Ach在早期成熟大神经元中具有神经营养作用的概念。(3)与形成较晚、分布更广的其他神经传递系统相比，大小脑胆碱能网络似乎相对完好，不受营养不良的影响，甚至在更大程度上受甲状腺功能减退的影响。突触前间隔似乎比突触后间隔受影响更大。(4)与文献报道不一致的是，甲状腺和营养状态异常引起的异常是不可逆的。
To overcome the deficiencies of previous findings, the activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) were studied at very short age intervals to allow a more precise definition of the shape and timing of their developmental curves in normal, hypothyroid and underfed rats. In addition, AchE expression in developing cerebellum was studied histochemically in these three neurological models. When compared with structural findings in the literature, the results provide the following information on the normal and abnormal developing cholinergic system, related or not to cerebellar neurotransmission (1) AchE activity, unlike ChAT, can be considered as a good marker of the developing cholinergic archicerebellum, (2) ChAT and AchE are transiently expressed together in functionally noncholinergic Purkinje cells. In contrast with most regions of the central nervous system, the high ratio of ChAT to AchE activities in the early stage of cerebellar development suggests an enhanced synthesis of acetylcholine (Ach). The level of ChAT activity correlates with Purkinje cell size, supporting the concept of a neurotrophic role of Ach in early maturing macroneurons. (3) The archicerebellar cholinergic network appears to be relatively well preserved from undernutrition and, to an even greater extent, from hypothyroidism, compared to other systems of neurotransmission formed later and more widely distributed throughout the cerebellum. The presynaptic compartment seems to be more affected than the postsynaptic compartment. (4) In disagreement with some data in the literature, the abnormalities induced by both abnormal thyroidal and nutritional states were found to be irreversible.