Cnidarian-Symbiodiniaceae symbiosis establishment is independent of photosynthesis
Cnidarian-Symbiodiniaceae symbiosis establishment is independent of photosynthesis
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DOI：
10.1016/j.cub.2022.04.021
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发表时间：
2022-06-06
期刊：
影响因子：
9.2
通讯作者：
Xiang, Tingting
中科院分区：
文献类型：
--
作者：
Jinkerson, Robert E.;Russo, Joseph A.;Xiang, Tingting
光合作用塑造了刺胞动物和共生藻之间的共生关系，许多刺胞动物宿主需要共生体光合作用才能生存，但人们对光合作用如何影响共生关系的建立知之甚少。在这里，我们表明，在共生的建立，感染，增殖和维护可以进行没有光合作用，但这样做的能力是依赖于特定的刺胞藻共生关系。对31对共生关系（海葵，珊瑚和水母宿主中的5种共生菌科）的评估表明，感染可以在没有光合作用的情况下发生。建立了共生藻的紫外诱变方法，并利用该方法获得了6个能侵染这些寄主的光合突变体。没有光合作用，共生藻科不能在海葵Aiptasia或水母Cassiopea中增殖，但可以在珊瑚Acropora的幼年息肉中增殖。经过6个月的黑暗，Breviolum minutum维持在Aiptasia内，表明共生藻科的维持可以独立于光合作用。操纵光合作用提供了深入了解刺胞动物共生。
Photosynthesis shapes the symbiotic relationships between cnidarians and Symbiodiniaceae algae-with many cnidarian hosts requiring symbiont photosynthate for survival-but little is known about how photosynthesis impacts symbiosis establishment. Here, we show that during symbiosis establishment, infection, proliferation, and maintenance can proceed without photosynthesis, but the ability to do so is dependent on specific cnidarian-Symbiodiniaceae relationships. The evaluation of 31 pairs of symbiotic relationships (five species of Symbiodiniaceae in sea anemone, coral, and jellyfish hosts) revealed that infection can occur without photosynthesis. A UV mutagenesis method for Symbiodiniaceae was established and used to generate six photosynthetic mutants that can infect these hosts. Without photosynthesis, Symbiodiniaceae cannot proliferate in the sea anemone Aiptasia or jellyfish Cassiopea but can proliferate in the juvenile polyps of the coral Acropora. After 6 months of darkness, Breviolum minutum is maintained within Aiptasia, indicating that Symbiodiniaceae maintenance can be independent of photosynthesis. Manipulating photosynthesis provides insights into cnidarian-Symbiodiniaceae symbiosis.