B-1-like cells exist in sheep.: Characterization of their phenotype and behaviour
B-1-like cells exist in sheep.: Characterization of their phenotype and behaviour
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DOI：
10.1046/j.1365-2567.1998.00599.x
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发表时间：
1998-10-01
期刊：
影响因子：
6.4
通讯作者：
Schwartz-Cornil, I
中科院分区：
文献类型：
--
作者：
Chevallier, N;Berthelemy, M;Schwartz-Cornil, I
已经描述了两个B淋巴细胞群体，B-1（CD 5（+）和/或CD 11 B（+））和B-2（CD 5（-）和CD 11 B（-））细胞。在作为B-1和B-2细胞研究的参照物种的小鼠中，这两个亚群呈现不同的发育方案、表型、抗体库、定位和行为。有趣的是，在绵羊中，B细胞在新生期前后重排其免疫球蛋白（IG）基因座，类似于鼠B-1细胞。然而，绵羊B细胞的表型尚未就其发育途径进行表征。在这份报告中，我们表明，两个绵羊B细胞亚群可以区分的基础上CD 11b的表达。相对于CD 11 B（-）B细胞，CD 11 B（+）B细胞通常共表达CD 5、CD 11 c、更高水平的表面IgM（sIgM），显示更大的细胞尺寸和更高的细胞循环活性，并因此呈现B-1样表型。然而，与鼠B-1细胞不同，绵羊B-1样细胞主要定位于血液中，相对于B-2样细胞显示出更高的自发凋亡倾向，并且在sIgM刺激后增殖。我们的数据显示，尽管新生儿免疫球蛋白基因座重排，绵羊B细胞并不都表达B-1样表型。然而，B-1样细胞和B-2样细胞共存并呈现表型和行为特异性。然而，绵羊B-1样细胞和B-2样细胞与鼠B-1样细胞和鼠B-2样细胞不同。细胞的行为。因此，这些子集不能被视为物种之间的真正同源物。
Two populations of B lymphocytes, B-l (CD5(+) and/or CD11b(+)) and B-2 (CD5(-) and CD11b(-)) cells have been described. In mice, which is the species of reference for B-l and B-2 cell studies, these two subsets present different developmental schemes, phenotypes, antibody repertoires, localization and behaviours. Interestingly, in sheep, B cells rearrange their immunoglobulin (Ig) loci around the neonatal period, similarly to murine B-l cells. However, the phenotype of the sheep B cells has not been characterized with regards to their developmental pathway. In this report, we show that two sheep B-cell subsets can be distinguished on the basis of CD11b expression. Relative to CD11b(-) B cells, the CD11b(+) B cells frequently co-express CD5, CD11c, higher levels of surface IgM (sIgM), show larger cell size and higher cell-cycling activity, and thus present a B-l-like phenotype. However, unlike murine B-l cells, sheep B-l like cells mainly localize in blood, display a higher propensity to spontaneous apoptosis relative to B-2-like cells, and proliferate after sIgM stimulation. Our data show that despite neonatal immunoglobulin loci rearrangements, sheep B cells do not all express a B-l-like phenotype. However, B-l-and B-2-like cells co-exist and present phenotypic and behavioural specificities. Nevertheless, sheep B-l-and B-2-like cells differ from the murine B-l and B-2. cells in their cell behaviour. These subsets can thus not be considered as true homologues among species.