Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
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DOI：
10.3389/fpls.2019.01074
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发表时间：
2019-09
影响因子：
5.6
通讯作者：
C. Paetzold;K. R. Wood;Deren A. R. Eaton;W. L. Wagner;M. Appelhans
中科院分区：
文献类型：
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作者：
C. Paetzold;K. R. Wood;Deren A. R. Eaton;W. L. Wagner;M. Appelhans
夏威夷Melicope是夏威夷群岛的主要适应性辐射之一，由54种特有物种组成。谱系是单系的，估计树冠年龄早于当前高岛的崛起。基于Sanger测序的系统发育推断还不足以解决物种或更深层次的关系。在这里，我们应用限制性内切位点相关DNA测序（RAD-seq）来推断谱系的系统发育关系。我们采用四重奏抽样来评估信息内容和统计支持，并量化不一致以及分割ABBA-BABA测试，以发现渗入的证据。我们的新结果大大提高了夏威夷Melicope内部关系的分辨率。谱系被划分为五个完全支持的主枝，其中两个对应于形态学上受限的内支群。我们提供了祖先和现在杂交事件的证据。我们确认有必要对Melicope Pelea部分进行分类修订，并结合基因组和形态学数据对几个物种复合体进行重新评估。
Hawaiian Melicope are one of the major adaptive radiations of the Hawaiian Islands comprising 54 endemic species. The lineage is monophyletic with an estimated crown age predating the rise of the current high islands. Phylogenetic inference based on Sanger sequencing has not been sufficient to resolve species or deeper level relationships. Here, we apply restriction site-associated DNA sequencing (RAD-seq) to the lineage to infer phylogenetic relationships. We employ Quartet Sampling to assess information content and statistical support, and to quantify discordance as well as partitioned ABBA-BABA tests to uncover evidence of introgression. Our new results drastically improved resolution of relationships within Hawaiian Melicope. The lineage is divided into five fully supported main clades, two of which correspond to morphologically circumscribed infrageneric groups. We provide evidence for both ancestral and current hybridization events. We confirm the necessity for a taxonomic revision of the Melicope section Pelea, as well as a re-evaluation of several species complexes by combining genomic and morphological data.