INTERACTION OF BEE MELITTIN WITH LIPID BILAYER MEMBRANES
INTERACTION OF BEE MELITTIN WITH LIPID BILAYER MEMBRANES
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DOI：
10.1016/0005-2736(78)90131-1
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发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
HIDER, RC
中科院分区：
文献类型：
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作者：
DAWSON, CR;DRAKE, AF;HIDER, RC
据报道，蜂毒肽（来自蜜蜂毒液）和相关的 8-26 肽对双层脂膜的稳定性和电性能的影响。与 8-26 肽不同，蜂毒肽对脂质膜具有显着影响，在稀浓度下会导致脂质膜破裂。蜂毒肽的圆二色性表明，在生理条件下，在水中，蜂毒肽处于延伸构象，在乙醇水溶液中这种构象得到增强。然而，在类似膜的条件下，它本质上是α-螺旋的。使用二级结构预测来定位可能的α-螺旋成核中心，并根据这些预测建立蜂毒肽模型。据推测，蜂毒肽会在膜中引起楔形效应。
The influence of melittin [from honeybee venom] and the related 8-26 peptide on the stability and electrical properties of bilayer lipid membranes is reported. Melittin, unlike the 8-26 peptide, has a dramatic influence on lipid membranes, causing rupture at dilute concentrations. The circular dichroism of melittin demonstrated that under physiological conditions, in water, melittin is in extended conformation, which is enhanced in aqueous ethanol. However in membrane-like conditions it is essentially .alpha.-helical. Secondary structure predictions were used to locate possible .alpha.-helical nucleation centers and a model of melittin was built according to these predictions. It is postulated that melittin causes a wedge effect in membranes.